Protein Info for MIT1002_00653 in Alteromonas macleodii MIT1002

Annotation: putative diguanylate cyclase AdrA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 329 PF08448: PAS_4" amino acids 24 to 145 (122 residues), 24.1 bits, see alignment E=3.6e-09 TIGR00254: diguanylate cyclase (GGDEF) domain" amino acids 159 to 322 (164 residues), 186.9 bits, see alignment E=1.2e-59 PF00990: GGDEF" amino acids 164 to 319 (156 residues), 167 bits, see alignment E=3.1e-53

Best Hits

KEGG orthology group: None (inferred from 88% identity to amc:MADE_03704)

Predicted SEED Role

"diguanylate cyclase (GGDEF domain) with PAS/PAC sensor"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (329 amino acids)

>MIT1002_00653 putative diguanylate cyclase AdrA (Alteromonas macleodii MIT1002)
MNASTDALSTGALVNSSQVPAELLDSIEVGIAVLDRNFNVQVWNKFLENHGAKKAEAIIG
DSLFSHFPEIEEKWLRTKVDPVFNLKSPVFIIWEQRPYLFKFGCNRPVTSAAEFMYQNVT
MFPIVDKRGNVERFCMLVYDVTEQALGKLGMEHLNEELKTASRVDGLTGLYNRRYWQERF
DEMHKLCVRREKPSTALMLDIDHFKRINDTYGHQAGDKVIKMLAALIKRCVRETDLAGRY
GGEEFAIILNDSSVEDAKIVAERIRQLAQRLVVEHEGESISFTVSLGLAQFSPDFKGAMA
WLECADQALYEAKENGRNQYRIYAAPGAL