Protein Info for MIT1002_00639 in Alteromonas macleodii MIT1002

Annotation: putative efflux pump membrane fusion protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 492 signal peptide" amino acids 1 to 31 (31 residues), see Phobius details TIGR01730: efflux transporter, RND family, MFP subunit" amino acids 111 to 423 (313 residues), 107.2 bits, see alignment E=4.2e-35 PF13437: HlyD_3" amino acids 252 to 312 (61 residues), 27 bits, see alignment E=3.2e-10

Best Hits

Predicted SEED Role

"Membrane fusion protein of RND family multidrug efflux pump" in subsystem Multidrug Resistance Efflux Pumps

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (492 amino acids)

>MIT1002_00639 putative efflux pump membrane fusion protein (Alteromonas macleodii MIT1002)
MRNKRIIVTLLAAASIALAVMYTMMNMGAQHPGAKPQTESKPTSPVISANAEQSQNAAQA
KKPAGGERPAALPTADSGVNRLNKASDTSSVNTTNRSPKGEQSGNTQLAQVGVIDVTADS
YNAKVRGYGQVVPQDSLSLVAQVSGQVTDISSSFKTGALVANNTVLARIDDTNYQQALAS
ANATYQAAVVSLEEERLQGIQAKDEWTRSGLDGEPLSALVLREPQLKAAQATLDEAEQSV
NSAKRDLAFTSLRAPFNALVVSRDIALGSYVQAGSAVATLYSADIAEVAIGLSPSQWAQL
PSGDETQLSGDASLNWPVTLTDTTTGNTWVANIVRAEWHQSDTTRQRNAIAVVEKPLAYS
TPLLFGSFVQADIEGRALDNVWKLPASAISQKQEVWLVMPSTGQLAKFTPSVLFESEGYA
YITPPKTSEVTGDIVSNVAGKTSGNASGNSMSEQVGEIRQALVVARPLNSYLVNTKVLPV
VEGQTGGQADDK