Protein Info for MIT1002_00631 in Alteromonas macleodii MIT1002

Annotation: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01128: IspD
  • amino acids 6 to 227 (222 residues), 265 bits, E=6.1e-83
• TIGR00453: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase
  • amino acids 7 to 224 (218 residues), 239.1 bits, E=1.8e-75
• PF12804: NTP_transf_3
  • amino acids 8 to 127 (120 residues), 51.3 bits, E=1.6e-17

Best Hits

Swiss-Prot: 84% identical to ISPD_ALTMD: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (ispD1) from Alteromonas mediterranea (strain DSM 17117 / CIP 110805 / LMG 28347 / Deep ecotype)

KEGG orthology group: K00991, 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase [EC: 2.7.7.60] (inferred from 84% identity to amc:MADE_03721)

MetaCyc: 57% identical to 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (Escherichia coli K-12 substr. MG1655)
2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase. [EC: 2.7.7.60]

Predicted SEED Role

"2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (EC 2.7.7.60)" in subsystem Isoprenoid Biosynthesis or Teichoic and lipoteichoic acids biosynthesis or polyprenyl synthesis (EC 2.7.7.60)

MetaCyc Pathways

• superpathway of geranylgeranyl diphosphate biosynthesis II (via MEP) (11/12 steps found)
• taxadiene biosynthesis (engineered) (11/13 steps found)
• methylerythritol phosphate pathway I (8/9 steps found)
• methylerythritol phosphate pathway II (8/9 steps found)
• isoprene biosynthesis I (8/10 steps found)
• superpathway of ergosterol biosynthesis II (10/26 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from terpenoid and polyketide
• Biosynthesis of plant hormones
• Biosynthesis of terpenoids and steroids
• Terpenoid biosynthesis

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.7.60

Protein Sequence (253 amino acids)

>MIT1002_00631 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (Alteromonas macleodii MIT1002)
MTSPRVVAVIPAAGVGSRMQADRPKQYLTLNGKTILEHTIDALLQHPLIDDVIVAISPGD
AYFDQYRLREKPIRVVDGGKERADSVLNGLMSLGENDWALVHDAARPCIEESDISALLEL
MSSDDVSGGILATPVRDTMKRVRPSTNVISHTEDRDGLWHALTPQLFPATLLKRALQNGL
SQGANITDEASAMELEGYKVAMVSGSPANIKITHPADLPLAEFYLKQKLSAQGQNAQNYR
VQSHSAKNQKAQS