Protein Info for MIT1002_00628 in Alteromonas macleodii MIT1002

Annotation: Protein-L-isoaspartate O-methyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 211 TIGR00080: protein-L-isoaspartate O-methyltransferase" amino acids 5 to 210 (206 residues), 252.1 bits, see alignment E=2.5e-79 PF01135: PCMT" amino acids 8 to 209 (202 residues), 229.5 bits, see alignment E=5.9e-72 PF13649: Methyltransf_25" amino acids 82 to 167 (86 residues), 27.1 bits, see alignment E=8.2e-10

Best Hits

Swiss-Prot: 100% identical to PIMT_ALTMD: Protein-L-isoaspartate O-methyltransferase (pcm) from Alteromonas mediterranea (strain DSM 17117 / CIP 110805 / LMG 28347 / Deep ecotype)

KEGG orthology group: K00573, protein-L-isoaspartate(D-aspartate) O-methyltransferase [EC: 2.1.1.77] (inferred from 100% identity to amc:MADE_03724)

MetaCyc: 59% identical to protein-L-isoaspartate O-methyltransferase (Escherichia coli K-12 substr. MG1655)
Protein-L-isoaspartate(D-aspartate) O-methyltransferase. [EC: 2.1.1.77]

Predicted SEED Role

"Protein-L-isoaspartate O-methyltransferase (EC 2.1.1.77)" in subsystem Ton and Tol transport systems (EC 2.1.1.77)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.1.1.77

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (211 amino acids)

>MIT1002_00628 Protein-L-isoaspartate O-methyltransferase (Alteromonas macleodii MIT1002)
MRASRKGDALAQLLYNEGIRSQAVLNAIAGTPRESFLPDALKHKAYQNTALPIGQGQTIS
QPYIVAKMTELLLDSPNKPEKVLEIGTGSGYQTAILAQLFAKVFSVERIKSLQFQAKRRM
NQLDLHNIAMKHGDGWKGWASKGPYDAIIVTAAAASLPQDLCDQLKEGGRLIIPVGNEQQ
SLLCIDRIEGELKTSTIESVRFVPLVAGELM