Protein Info for MIT1002_00514 in Alteromonas macleodii MIT1002

Annotation: Guanosine-5'-triphosphate,3'-diphosphate pyrophosphatase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 503 PF02541: Ppx-GppA" amino acids 29 to 310 (282 residues), 267.8 bits, see alignment E=1.2e-83 PF21447: Ppx-GppA_III" amino acids 317 to 489 (173 residues), 149 bits, see alignment E=1.4e-47

Best Hits

Swiss-Prot: 65% identical to GPPA_PSEA6: Guanosine-5'-triphosphate,3'-diphosphate pyrophosphatase (gppA) from Pseudoalteromonas atlantica (strain T6c / ATCC BAA-1087)

KEGG orthology group: K01524, exopolyphosphatase / guanosine-5'-triphosphate,3'-diphosphate pyrophosphatase [EC: 3.6.1.11 3.6.1.40] (inferred from 92% identity to amc:MADE_00567)

Predicted SEED Role

"Guanosine-5'-triphosphate,3'-diphosphate pyrophosphatase (EC 3.6.1.40)" in subsystem Phosphate metabolism or Stringent Response, (p)ppGpp metabolism (EC 3.6.1.40)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.6.1.11, 3.6.1.40

Use Curated BLAST to search for 3.6.1.11 or 3.6.1.40

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (503 amino acids)

>MIT1002_00514 Guanosine-5'-triphosphate,3'-diphosphate pyrophosphatase (Alteromonas macleodii MIT1002)
MQAELQHDAQTYGEYYAAVDLGSNSFHMVIVHVVNGSVQIIGKIKQKVRLAAGLDDELAL
DDVSMERGWQCLQVFSERLQDIPPSNIKIVATATLRLATNAHVFIRKAEEILNHKLEVIS
GEEEARQIYLGVAYTSANQGNSLVIDIGGASTEIIIGNDMQPIHLKSLDMGCVTFMERYF
TGSTITEDNFDNAKQAAHALIADVSSAFLCFDWENCLGASGTPQAITEILVAQGISDAIR
LDYLYHLEKQCIECGSIKTLDIDGLEDNRKAIFPSGLAILISLFEALNIKHMNISGGALR
EGLIYGMLDNVTENDRRQQTLNQAKHRYHVDASHANSVSHVALTLCHQLCAQQNICHLDT
ETILGAAAQLHEIGLHIEYKRHHEHGEYILSHIDLPGFTRLQRAAIRDLVAQHRLNIKPD
VFEKYHEDYRPMLKGLLRILRIAVVLCLRRQNKHIPEVTLQCEESKWTLTFPEGWLKSHP
LINAELANECWLQHKAGCELVCK