Protein Info for MIT1002_00455 in Alteromonas macleodii MIT1002

Annotation: NAD-dependent malic enzyme

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00390: malic
  • amino acids 90 to 270 (181 residues), 259.8 bits, E=1.4e-81
• PF03949: Malic_M
  • amino acids 280 to 539 (260 residues), 340.8 bits, E=4.7e-106

Best Hits

Swiss-Prot: 74% identical to MAO1_IDILO: NAD-dependent malic enzyme (maeA) from Idiomarina loihiensis (strain ATCC BAA-735 / DSM 15497 / L2-TR)

KEGG orthology group: K00027, malate dehydrogenase (oxaloacetate-decarboxylating) [EC: 1.1.1.38] (inferred from 97% identity to amc:MADE_00517)

MetaCyc: 66% identical to malate dehydrogenase (oxaloacetate-decarboxylating) (Escherichia coli K-12 substr. MG1655)
Malate dehydrogenase (oxaloacetate-decarboxylating). [EC: 1.1.1.38]

Predicted SEED Role

"NAD-dependent malic enzyme (EC 1.1.1.38)" in subsystem Pyruvate metabolism I: anaplerotic reactions, PEP (EC 1.1.1.38)

MetaCyc Pathways

• superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass (23/26 steps found)
• gluconeogenesis I (13/13 steps found)
• superpathway of cytosolic glycolysis (plants), pyruvate dehydrogenase and TCA cycle (19/22 steps found)
• TCA cycle III (animals) (9/10 steps found)
• glyoxylate cycle (6/6 steps found)
• TCA cycle II (plants and fungi) (8/9 steps found)
• mixed acid fermentation (13/16 steps found)
• superpathway of glyoxylate bypass and TCA (10/12 steps found)
• superpathway of glyoxylate cycle and fatty acid degradation (11/14 steps found)
• TCA cycle I (prokaryotic) (8/10 steps found)
• TCA cycle VIII (Chlamydia) (5/6 steps found)
• malate/L-aspartate shuttle pathway (2/2 steps found)
• TCA cycle V (2-oxoglutarate synthase) (7/9 steps found)
• gluconeogenesis III (9/12 steps found)
• L-malate degradation II (1/1 steps found)
• chitin deacetylation (3/4 steps found)
• C4 photosynthetic carbon assimilation cycle, NAD-ME type (8/11 steps found)
• anaerobic energy metabolism (invertebrates, cytosol) (5/7 steps found)
• TCA cycle IV (2-oxoglutarate decarboxylase) (6/9 steps found)
• L-carnitine degradation II (1/3 steps found)
• formaldehyde assimilation I (serine pathway) (8/13 steps found)
• reductive TCA cycle I (6/11 steps found)
• incomplete reductive TCA cycle (3/7 steps found)
• anaerobic energy metabolism (invertebrates, mitochondrial) (5/10 steps found)
• superpathway of anaerobic energy metabolism (invertebrates) (10/17 steps found)
• methylgallate degradation (2/6 steps found)
• protocatechuate degradation I (meta-cleavage pathway) (3/8 steps found)
• pyruvate fermentation to propanoate I (2/7 steps found)
• reductive TCA cycle II (5/12 steps found)
• superpathway of vanillin and vanillate degradation (3/10 steps found)
• methylaspartate cycle (9/19 steps found)
• (S)-lactate fermentation to propanoate, acetate and hydrogen (4/13 steps found)
• syringate degradation (3/12 steps found)
• L-glutamate degradation VIII (to propanoate) (2/11 steps found)
• Methanobacterium thermoautotrophicum biosynthetic metabolism (21/56 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from terpenoid and polyketide
• Biosynthesis of phenylpropanoids
• Biosynthesis of terpenoids and steroids
• Pyruvate metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.1.1.38

Protein Sequence (574 amino acids)

>MIT1002_00455 NAD-dependent malic enzyme (Alteromonas macleodii MIT1002)
MYLPEHIIGKPMSEDSQRYLYIPHAGPSLLETPLLNKGSAFTARERAAFNLTGLVPPRYE
TIEEQVERAYMQYSSFDEALNKHIYLRAIQDNNETLFYRLIQKHIDEMMPIIYTPTVGDA
CEQFSDIYRSSRGLFVSYEERHQLDDIVRNATKRKVKVIVVTDGERILGLGDQGIGGMGI
PIGKLSLYTACGGISPAYTLPVMLDVGTNNEKLLNDPMYMGARHPRIGQEEYDEFVDMFI
NAVKRRWPDVMIQFEDFAQPNAMPLLNRYRNDVCCFNDDIQGTAAVTLGTILAACKTKQQ
KLRDMKVVFVGAGSAGCGIAEMLIQQMVFEGLTDEQARKQVFMIDRFGLVTEGMEGLRDF
QQKLQQKNADLADWTFSGDYASLLDVMHCAQPDVLIGVSGQPGLFTEQVIRAMKQGCELP
IIFPLSNPSRQVEARPEQVIEWTEGEVIIATGSPFKPVEYDGKIFPIAQCNNSYIFPGIG
LGVVASKAKLISDEMLMAASNALASASPLVNTGQGSLLPPLAAIAEISREIAFEVGRVAM
EQGLALEISDDALRASIERNFWKAEYRPYKRVSI