Protein Info for MIT1002_00409 in Alteromonas macleodii MIT1002

Annotation: Murein polymerase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 783 transmembrane" amino acids 32 to 50 (19 residues), see Phobius details amino acids 692 to 708 (17 residues), see Phobius details TIGR02071: penicillin-binding protein 1B" amino acids 33 to 750 (718 residues), 881.1 bits, see alignment E=2.8e-269 PF14814: UB2H" amino acids 78 to 161 (84 residues), 65.6 bits, see alignment E=5e-22 PF00912: Transgly" amino acids 172 to 343 (172 residues), 191.1 bits, see alignment E=1.7e-60 PF00905: Transpeptidase" amino acids 436 to 695 (260 residues), 76 bits, see alignment E=4.5e-25

Best Hits

Swiss-Prot: 44% identical to PBPB_VIBCH: Penicillin-binding protein 1B (mrcB) from Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)

KEGG orthology group: K05365, penicillin-binding protein 1B [EC: 2.4.1.129 3.4.-.-] (inferred from 96% identity to amc:MADE_00472)

MetaCyc: 42% identical to peptidoglycan glycosyltransferase / peptidoglycan DD-transpeptidase MrcB (Escherichia coli K-12 substr. MG1655)
Serine-type D-Ala-D-Ala carboxypeptidase. [EC: 3.4.16.4]; 3.4.16.4 [EC: 3.4.16.4]; Peptidoglycan glycosyltransferase. [EC: 3.4.16.4, 2.4.1.129]

Predicted SEED Role

"Multimodular transpeptidase-transglycosylase (EC 2.4.1.129) (EC 3.4.-.-)" in subsystem Peptidoglycan Biosynthesis or Type IV pilus (EC 2.4.1.129, EC 3.4.-.-)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.4.1.129, 3.4.-.-, 3.4.16.4

Use Curated BLAST to search for 2.4.1.129 or 3.4.-.- or 3.4.16.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (783 amino acids)

>MIT1002_00409 Murein polymerase (Alteromonas macleodii MIT1002)
MAKRQTKDNPSSKSKASSNGKGSAKRSWFFKLFWRVTLIGLVVLGAYAFYLDAQIKHAFS
GNKWEVPAQIFARPLEISKGEEITPQEVIDELNLLGYRKVTFADSSGEYSYQNKVLTVQR
RAFQFPEGAEPLRHIVITWEGARIGEIYDKSQGRPYGSAKLEPWLVTRMTSGSQEDRMLV
TLDTIPDMLPKALTLVEDRNFYNHHGVAPLSILRALMANIAAGRTVQGGSTLTQQLVKNM
FLTRERSIVRKAKEAIMAVIIDARYSKSEIIEAYLNEVFLGQNGDMAVHGFGLASYFYFD
RPANELSTPEIATLVAIIKGPSYYNPRKYAERTKERRDLVLRMLFDENEISRQDYERFVN
MPLGLASGASLASGKHPAFMEQVRRELNEILAEPSLRDSGVKVFTTLDIVAQRRAESALS
GTLDGLQKDRKVPLQGAMVVTDSASGEVRAIVGGKDFAFKGFNRALDAKRPIGSLIKPAV
YLTALEDPTQFNLATPLKDEPITLESRNGKTWSPQNYDKEFRGQVPLLQGLVESLNIPTV
NLGMQIGLDALADTIERLGVTTPVDKVPSLTLGAFELTPFSTNQMYQTLSNDGRYIPLHT
VTSVVTADNALLWKKAEFFAQRTDEAATYLLNYALYKVTTEGTAKRIGWNFGNVNMAGKT
GTTDDYRDSWFSGFDRNNVVTVWVGNDDNQPVYLTGAGGALPVFINYMKSQSPKSLSRRF
PKGLGIAHFDAKTGAVVVAGCAGSLSVPAILDVLPPPQQDCSGRPVDKDSDKDDKSWWER
IFG