Protein Info for MIT1002_00406 in Alteromonas macleodii MIT1002

Annotation: ATP-dependent RNA helicase HrpB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 880 TIGR01970: ATP-dependent helicase HrpB" amino acids 8 to 879 (872 residues), 750.5 bits, see alignment E=1.3e-229 PF00270: DEAD" amino acids 20 to 164 (145 residues), 44.6 bits, see alignment E=2e-15 PF00271: Helicase_C" amino acids 213 to 335 (123 residues), 45.8 bits, see alignment E=9.9e-16 PF08482: HrpB_C" amino acids 736 to 867 (132 residues), 150.1 bits, see alignment E=7.2e-48

Best Hits

KEGG orthology group: K03579, ATP-dependent helicase HrpB [EC: 3.6.4.13] (inferred from 79% identity to amc:MADE_00470)

Predicted SEED Role

"ATP-dependent helicase HrpB"

Isozymes

Compare fitness of predicted isozymes for: 3.6.4.13

Use Curated BLAST to search for 3.6.4.13

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (880 amino acids)

>MIT1002_00406 ATP-dependent RNA helicase HrpB (Alteromonas macleodii MIT1002)
MLQPFSHLPAYELVTPLEEAVAASNVIIGAPPGAGKSTVLPLSLLKSSLKGKILLMQPRR
VVVRNLADYLARQLNENVGETVGYRIKGESKTSSATRLEIITEGVLTRMIQQDPELDGVA
AIVFDEFHERSIHSDFGLALALEVQSGLRDDLRLVVMSATLDVAPLQTLLNAHSVLPVVN
LNTQGRMFAVDIRYTQDVQAHELVPKTCNIIKQAVGEHDGDILVFLPGRGSINAVAKEIK
GLAESADMAVHMLYGALSKSVQQAAITPDPQGRRKIILSTNIAETSLTIEGVTVVIDSLW
ENSAQYHPSSDITALTQQRISQASAIQRAGRAGRVMAGTCYRLCSKSSFERLAKYNAAQI
EKEDLSSFLLDTLAWGSSPDTLALLTKPTQAQQQVAQQKLQRVNAISQSSITPYGRALAQ
LPCQPHLAHLLLTVKQGIDGLCADDNDALRHAAPFVVALSESNISVGGATNVMDALLHAN
SNTRSQLTQQATRYARYVRNNDGKAQRFKPDINELDEQALGLCIAIAFPLQVGYRRSASD
KSAESSNTTNGKPKTGIVEYKLASGKGAELTAAGSASIFNESENGDGPWLAVLDGQLIKS
TVAIRLAQSVPESWLRLAFPDAFKEKRSVILNSHTGVMEARVSTGFYEITLNSTPVSGQE
KSREKAFWDDALCNAWFAYLDALPLSSWPLSDADWQWWRRVAMAGKLNLPQDKAFDEPDP
WPLSLSALVHQAKDSLAPVLKNCKNIKNLKQLPWKTALNNGLSWPQQDALATLLPTHVTI
PTGREASLDYRDNGDVVLSVKMPELYSQGTPLTIAGGRITVVCELLSPAGRPLQTTSDLA
AFWHGSYKEIQKEMKGRYPKHFWPDDPANASPTSKTKKHM