Protein Info for MIT1002_00330 in Alteromonas macleodii MIT1002

Annotation: Copper-exporting P-type ATPase A

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 747 transmembrane" amino acids 98 to 115 (18 residues), see Phobius details amino acids 127 to 144 (18 residues), see Phobius details amino acids 164 to 183 (20 residues), see Phobius details amino acids 194 to 213 (20 residues), see Phobius details amino acids 345 to 367 (23 residues), see Phobius details amino acids 374 to 396 (23 residues), see Phobius details amino acids 690 to 709 (20 residues), see Phobius details amino acids 715 to 736 (22 residues), see Phobius details PF00403: HMA" amino acids 12 to 66 (55 residues), 51.1 bits, see alignment 2.9e-17 TIGR01511: copper-translocating P-type ATPase" amino acids 144 to 740 (597 residues), 619 bits, see alignment E=1.7e-189 TIGR01525: heavy metal translocating P-type ATPase" amino acids 162 to 739 (578 residues), 625.8 bits, see alignment E=1.7e-191 TIGR01494: HAD ATPase, P-type, family IC" amino acids 207 to 707 (501 residues), 302.4 bits, see alignment E=9.3e-94 PF00122: E1-E2_ATPase" amino acids 230 to 410 (181 residues), 193 bits, see alignment E=6.8e-61 PF00702: Hydrolase" amino acids 428 to 644 (217 residues), 114 bits, see alignment E=2.4e-36

Best Hits

KEGG orthology group: K01533, Cu2+-exporting ATPase [EC: 3.6.3.4] (inferred from 100% identity to amc:MADE_00395)

Predicted SEED Role

"Lead, cadmium, zinc and mercury transporting ATPase (EC 3.6.3.3) (EC 3.6.3.5); Copper-translocating P-type ATPase (EC 3.6.3.4)" (EC 3.6.3.3, EC 3.6.3.4, EC 3.6.3.5)

Isozymes

Compare fitness of predicted isozymes for: 3.6.3.3, 3.6.3.4, 3.6.3.5

Use Curated BLAST to search for 3.6.3.3 or 3.6.3.4 or 3.6.3.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (747 amino acids)

>MIT1002_00330 Copper-exporting P-type ATPase A (Alteromonas macleodii MIT1002)
MSAEVTQRQLVIEGAGCASCVGKIEAALKQTPGVQNAEMNFADRTVLVSGTADSQLLVQA
VESVGYNAKPFEDKPDTDVVDEKEAADRTYYKKLMRDTFIALSLGIPLMTYSIVVDEMTV
ETNVERLLWLAVGLLTLGVMFFSGRHFYIGAWKSFKNHSANMDTLIALGTGTAWFYSMLV
VLVPEAVPLMARHVYFEATAMIIGLIDLGLALELKARGKTSEAIKRLIGLQAKTATVIRD
NKEVQVAIEQVLFNDVIKVRPGEKVPVDGVVVEGHTSIDESMLTGEPMPVEKSHDDEVVA
GTLNKSGMFLFKATRVGKDTALAQIIAMVKRAQNSKPPIGRLADVISAYFVPVVMIISVL
SALAWLNFGPEPALAFAIVSATTVLIIACPCALGLATPMSVMVGVGKAAEAGVLIRNGEA
LQTASKISVMILDKTGTITEGTPKVTDIILANFSDEKTVLQLAASLENGSEHPLAMAIVE
SAHDQGIELLKTEEFNAITGMGVEGRCEGKALLFGNSKLMASKGVGIGNYSEKAQTLAKD
AKTPMYFAVDGQLAAIIAVADPIKPDSVSAIKRLQANDIRVIMLTGDNKETAAAVAKKAA
ISEFFAEVLPEDKANKVHELQQQREVVGMTGDGINDAPALALADVGFAIGTGTDVAIESA
DITLMRGSLHGLADAIAVSKATLRNIKQNLFGAFIYNVAGIPFAAGVLYPFFGVLLSPVI
AGAAMAFSSLTVVSNANRLRLFKAKDH