Protein Info for MIT1002_00282 in Alteromonas macleodii MIT1002

Annotation: Hydrogenase isoenzymes formation protein HypE

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 TIGR02124: hydrogenase expression/formation protein HypE" amino acids 20 to 350 (331 residues), 362 bits, see alignment E=1.3e-112 PF00586: AIRS" amino acids 51 to 161 (111 residues), 72.9 bits, see alignment E=2.9e-24 PF02769: AIRS_C" amino acids 175 to 325 (151 residues), 55.9 bits, see alignment E=5.7e-19

Best Hits

KEGG orthology group: K04655, hydrogenase expression/formation protein HypE (inferred from 100% identity to amc:MADE_00353)

Predicted SEED Role

"[NiFe] hydrogenase metallocenter assembly protein HypE" in subsystem NiFe hydrogenase maturation

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (350 amino acids)

>MIT1002_00282 Hydrogenase isoenzymes formation protein HypE (Alteromonas macleodii MIT1002)
MKSAEGNNHSSSEPLGTESIQLAQGNGGTLTKRLIDHVFKNKANKDLDLLHDAATVTFDG
PYLSITTDSFVVSPPTFPGGNVGALSVYGTVNDLAVVGATPRYISTAFIIEEGFSLSTLK
QIVNGMHQAAEETKVAIVTGDTKVVPKGSGGGVFINTTGVGDSRDAPIWDTSLIKAGDHV
LVSGSVGDHGACVLLAREDYGLQGQLKSDCGSVVPLIDPIKHLEGVRFVRDPTRGGLSVL
LHDVANETGFDIELIENNIPVRPEVASVCEILGFDPFVLACEGRIVAVVAPDISGKVLEH
WRSIRNGEQAEHIGVIVKNTESKGRVTIVTPMGGRRFMNELEDEPLPRIC