Protein Info for MIT1002_00189 in Alteromonas macleodii MIT1002

Annotation: Galactokinase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 379 TIGR00131: galactokinase" amino acids 6 to 377 (372 residues), 424.6 bits, see alignment E=1.8e-131 PF10509: GalKase_gal_bdg" amino acids 9 to 57 (49 residues), 80.5 bits, see alignment 7.6e-27 PF00288: GHMP_kinases_N" amino acids 113 to 179 (67 residues), 65.8 bits, see alignment E=5.7e-22 PF08544: GHMP_kinases_C" amino acids 278 to 359 (82 residues), 43.7 bits, see alignment E=4.5e-15

Best Hits

Swiss-Prot: 58% identical to GAL1_PROMH: Galactokinase (galK) from Proteus mirabilis (strain HI4320)

KEGG orthology group: K00849, galactokinase [EC: 2.7.1.6] (inferred from 93% identity to amc:MADE_00232)

MetaCyc: 54% identical to galactokinase (Escherichia coli K-12 substr. MG1655)
Galactokinase. [EC: 2.7.1.6]

Predicted SEED Role

"Galactokinase (EC 2.7.1.6)" in subsystem Lactose and Galactose Uptake and Utilization (EC 2.7.1.6)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.1.6

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (379 amino acids)

>MIT1002_00189 Galactokinase (Alteromonas macleodii MIT1002)
MDNQVLSSNFETHYGEAPALIAHAPGRVNIIGEHTDYNEGFVFPAAINFGTWVAATKRAD
NDIVVTAMDYENQQNQFSLSDINYDEEQGWANYVRGVVKVLKEAMPDFGGANLLVTGNVP
QGAGLSSSASFEVAILKALSALYELPLDGVQAALLGQKAENTFVGCSCGIMDQLISAMGN
EGMAMLLDCQSLAIEHSPLPDSHQIVIINSNVKRGLVDSEYNLRRQQCEQGASLLGVSSL
REATMEMLEGAKAHMPEVVYRRAKHIVTENARTLAASQALKAGDIETVSEAMAQSHISMR
DDFEITVRPIDYLVEIIGEVLGKSGGVRMTGGGFGGCVVALVPTDKVEAVKQVVADKYSD
ETGYSADIYVCTATQGAFA