Protein Info for MIT1002_00153 in Alteromonas macleodii MIT1002

Annotation: DNA helicase II

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 723 TIGR01075: DNA helicase II" amino acids 6 to 721 (716 residues), 1194.2 bits, see alignment E=0 PF00580: UvrD-helicase" amino acids 10 to 272 (263 residues), 314.7 bits, see alignment E=2e-97 PF13245: AAA_19" amino acids 14 to 256 (243 residues), 81.3 bits, see alignment E=2.3e-26 PF13361: UvrD_C" amino acids 278 to 617 (340 residues), 279.7 bits, see alignment E=1.3e-86 PF13538: UvrD_C_2" amino acids 558 to 613 (56 residues), 35.1 bits, see alignment 3e-12 PF21196: PcrA_UvrD_tudor" amino acids 676 to 722 (47 residues), 31.9 bits, see alignment 3e-11

Best Hits

Swiss-Prot: 68% identical to UVRD_ECOLI: DNA helicase II (uvrD) from Escherichia coli (strain K12)

KEGG orthology group: K03657, DNA helicase II / ATP-dependent DNA helicase PcrA [EC: 3.6.4.12] (inferred from 99% identity to amc:MADE_00200)

MetaCyc: 68% identical to DNA helicase II (Escherichia coli K-12 substr. MG1655)
RXN-11135 [EC: 5.6.2.4]

Predicted SEED Role

"ATP-dependent DNA helicase UvrD/PcrA" in subsystem DNA repair, bacterial UvrD and related helicases

Isozymes

Compare fitness of predicted isozymes for: 3.6.4.12

Use Curated BLAST to search for 3.6.4.12 or 5.6.2.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (723 amino acids)

>MIT1002_00153 DNA helicase II (Alteromonas macleodii MIT1002)
MDVSRLLDELNDKQREAVAAPLSNALVLAGAGSGKTRVLVHRIAWLMEVENIAPFSILAV
TFTNKAAKEMRGRIESLMGRSLHNMWIGTFHGLAHRLLRAHHAEANLPENFQIIDSDDQY
RLIRRILKAMNLDEKHWAPRQIQWYINGNKDEGLRPQHIETHGDHIQKTMREVYAAYQDA
CDRSGLVDFAELLLRAHELWAKNPEVLAHYQRRFRAVLVDEFQDTNNIQYAWLRMLCSGG
NNNIMIVGDDDQSIYGWRGANVDNIQHFLKDFNEPTTIRLEQNYRSTGNILKAANTVIDN
NTGRLGKELWTEDAQGELISVYAGFNELDEARFIVSKIKDWLNQGNALTDTAILYRNNAQ
SRVLEEALLHEGLAYRIYGGLRFFERQEIKDALGYMRMVSHPHDDAAFERVVNTPSRGIG
EKTLSQVRDTARMHNCSMWQACQLLINEGGFKGRALNAMQSFVLLITELEQSTLDEPLDQ
QADKAIRQSGLYAMYQAERGEKAQARLENLEELVTACKTFIVPEEAEEMSPLAAFLAHAS
LEAGETQADKDQDAVQMMTIHTAKGLEFPLVFLAGVEEGMFPSQMTNDEPGRMEEERRLC
YVGMTRAMQKLYITYAESRRLYGQDKYHTASRFIREMPADCVEEVRLKSTISRPVHNRFS
QATSHASFEETGFSLGERVVHRKFGEGIVLNYEGSGEHGRVQVNFDEFGSKWLVLAYAKL
EKA