Protein Info for MIT1002_00148 in Alteromonas macleodii MIT1002

Annotation: ATP-dependent DNA helicase RecQ

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 613 TIGR01389: ATP-dependent DNA helicase RecQ" amino acids 22 to 607 (586 residues), 808.4 bits, see alignment E=3.7e-247 TIGR00614: ATP-dependent DNA helicase, RecQ family" amino acids 23 to 470 (448 residues), 568.8 bits, see alignment E=9.4e-175 PF04851: ResIII" amino acids 34 to 191 (158 residues), 26.6 bits, see alignment E=1.6e-09 PF00270: DEAD" amino acids 36 to 194 (159 residues), 99.7 bits, see alignment E=4.8e-32 PF00271: Helicase_C" amino acids 227 to 337 (111 residues), 80.7 bits, see alignment E=2.9e-26 PF16124: RecQ_Zn_bind" amino acids 349 to 410 (62 residues), 57.1 bits, see alignment E=7.5e-19 PF09382: RQC" amino acids 412 to 520 (109 residues), 116.1 bits, see alignment E=2.2e-37 PF00570: HRDC" amino acids 540 to 605 (66 residues), 74.2 bits, see alignment E=1.9e-24

Best Hits

Swiss-Prot: 55% identical to RECQ_ECOLI: ATP-dependent DNA helicase RecQ (recQ) from Escherichia coli (strain K12)

KEGG orthology group: K03654, ATP-dependent DNA helicase RecQ [EC: 3.6.4.12] (inferred from 97% identity to amc:MADE_00196)

MetaCyc: 55% identical to ATP-dependent DNA helicase RecQ (Escherichia coli K-12 substr. MG1655)
RXN-11135 [EC: 5.6.2.4]

Predicted SEED Role

"ATP-dependent DNA helicase RecQ" in subsystem DNA-replication or DNA repair, bacterial RecFOR pathway

Isozymes

Compare fitness of predicted isozymes for: 3.6.4.12

Use Curated BLAST to search for 3.6.4.12 or 5.6.2.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (613 amino acids)

>MIT1002_00148 ATP-dependent DNA helicase RecQ (Alteromonas macleodii MIT1002)
MTTAIAPSELAPETQRVKAPETVLKDVFGYDAFRDGQGEVIHQVCEGKDALVLLPTGGGK
SLCYQIPALVRQGTAIVVSPLISLMQDQVEQLKALGVKAAYLNSTLETDEQARINDALQA
GKLDLLYVSPERLMQYYFQQSLAHADIALFAIDEAHCVSHWGHDFRQDYRALGQIKSRFP
SIPVIGLTATADSATQADILTQLNLNDPLVYKGSFDRPNIRYRVMSKYKAFDQVVAYVKQ
QEGSGIIYCNSRAKVDDLHAKLFRQGFRCAAYHAGMDNDERELVQRQFLNDKIDIVVATV
AFGMGINKSNVRYVVHHDVPRSVESYYQETGRAGRDGLESEALLLFDEKDAARVKQWIEQ
GEIAERNQIELQKFAAMEAFSEAQTCRRQVLLNYFSQFSDSACGNCDICLDPPKMIDGLV
IAQKVLSCVLRLSQQASSQYVIDVLRGKQLRRLQEAGHHQLSTYGIGKDKSDSYWHNIIN
QLVHKGLIRVDITAHAALRLTEAARPVLKGEVAVQLAVPRLEFKPDKKKAKQAPANYDRT
LFTRLKHLRKVLAEENEVPPYVVFSDATLVDMACKLPTTRNTLLDVSGVGQTKLERYGEA
FIQLIDDYINREH