Protein Info for MIT1002_00145 in Alteromonas macleodii MIT1002

Annotation: Cyclic di-GMP phosphodiesterase Gmr

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 946 signal peptide" amino acids 1 to 27 (27 residues), see Phobius details transmembrane" amino acids 182 to 203 (22 residues), see Phobius details amino acids 213 to 238 (26 residues), see Phobius details amino acids 245 to 263 (19 residues), see Phobius details amino acids 275 to 294 (20 residues), see Phobius details amino acids 299 to 318 (20 residues), see Phobius details amino acids 325 to 346 (22 residues), see Phobius details amino acids 358 to 378 (21 residues), see Phobius details PF07696: 7TMR-DISMED2" amino acids 66 to 171 (106 residues), 25.5 bits, see alignment E=2.3e-09 TIGR00229: PAS domain S-box protein" amino acids 398 to 516 (119 residues), 57.6 bits, see alignment E=1.4e-19 PF13426: PAS_9" amino acids 419 to 512 (94 residues), 39.8 bits, see alignment E=9.6e-14 TIGR00254: diguanylate cyclase (GGDEF) domain" amino acids 520 to 681 (162 residues), 124.2 bits, see alignment E=4.3e-40 PF00990: GGDEF" amino acids 524 to 679 (156 residues), 117.9 bits, see alignment E=7.7e-38 PF00563: EAL" amino acids 702 to 937 (236 residues), 190.7 bits, see alignment E=5.5e-60

Best Hits

KEGG orthology group: None (inferred from 85% identity to amc:MADE_00193)

Predicted SEED Role

"diguanylate cyclase/phosphodiesterase (GGDEF & EAL domains) with PAS/PAC sensor(s)" in subsystem Bacterial hemoglobins

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (946 amino acids)

>MIT1002_00145 Cyclic di-GMP phosphodiesterase Gmr (Alteromonas macleodii MIT1002)
MPISQLTKYVAALLISWVVLSINAFASPRLVIDESFEREIINHYARVSIAPNSATYFDVL
TGSSTKTPTATASSTERVWYSTELVHSGYSAIPLVLNIDRLNVDDLQIYLLDSSQRIIKS
YRYQAGKGDYSLRQPLPNIRLAFTLQPYQDARLLIAVKDEGLKYFPMSLWERDLLAQHDT
NMLTLLGAVSGVMALIASYFLFSYLCQRIPTRFWLTMSSLVLIALLFITEGGLAFWPSLT
NASEHFYAVCFGVLLLCIGKVTHHLFSRVPFVLRLLNYALPLAATIYCFTVNAYMVTITL
LILTAAMGLYHVTLALIFKDKDSSVVSTTYILAWLSFFAFYALVTQNLFSDLIYTVDVAM
GMLFFLTLGFLCFGFAVITKEQVLNQHKLSSQAETISSLNHFYDLFRNSAEGHYTSTWDG
KLISVNPAMCKVFGYENEEEMLSEGASTKAFYASPEDRHVLLGEISQQGHVTRKEIRGKR
RDGTEFWFSLSCQIRENDEGTYLYGSIIDITEKKQSDLSLQYLATHDSLTGVYNRRQFES
VFKEKVHNHSALPVCILFLDLDRFKVVNDTCGHKAGDVLIKDVARLIENTLLPNAQLARL
GGDEFGVIYSDLDADAVYLNAVKILNAVQAYRFMWDNRIFNLGVSIGMVVCDDSNVSSGQ
YLSMADAACYFAKEQGRNQVHKYNKDDENMQRYQRELDWVSSINNALEEGRFELYYQSLR
PLSKASDGHYYEVLLRLREKDGKIVEPANFLPTAERFEMNVNVDKWVVTNTFKWLSENPE
HLAELRRCSINLNCHSLADRDFTLFILNAFETFNIPYNKICFEVIESVAIIKMEDTLAFM
RTFNRLGCSFALDDFGSGFSSYSYLKSLPVNQVKIDGMFIKDMLNDSVDTAMVASINDVA
KAMGMQTVGEFVENEGTMAQLGKMGVDFAQGYSVAKPAPLREFTPL