Protein Info for MIT1002_00109 in Alteromonas macleodii MIT1002

Annotation: Putative reactive intermediate deaminase TdcF

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00004: reactive intermediate/imine deaminase
  • amino acids 3 to 124 (122 residues), 167.1 bits, E=9.2e-54
• PF01042: Ribonuc_L-PSP
  • amino acids 9 to 123 (115 residues), 132.3 bits, E=4.8e-43

Best Hits

Swiss-Prot: 67% identical to YVN1_AZOVI: RutC family protein in vnfA 5'region from Azotobacter vinelandii

KEGG orthology group: K07567, TdcF protein (inferred from 98% identity to amc:MADE_00156)

MetaCyc: 47% identical to 2-iminobutanoate/2-iminopropanoate deaminase monomer (Salmonella enterica enterica serovar Typhimurium str. LT2)
Tryptophanase. [EC: 3.5.99.10, 4.1.99.1, 4.1.99.2, 4.3.1.13, 4.3.1.17, 4.3.1.18, 4.3.3.8, 4.4.1.1, 4.4.1.13, 4.4.1.15, 4.4.1.25, 4.4.1.28, 4.4.1.35, 4.5.1.2]; 3.5.99.10,4.3.1.-,4.3.1.19,4.4.1.1,4.4.1.1,4.4.1.2,3.5.99.7,4.4.1.11 [EC: 3.5.99.10, 4.1.99.1, 4.1.99.2, 4.3.1.13, 4.3.1.17, 4.3.1.18, 4.3.3.8, 4.4.1.1, 4.4.1.13, 4.4.1.15, 4.4.1.25, 4.4.1.28, 4.4.1.35, 4.5.1.2, 3.5.99.7, 4.3.1.19, 4.4.1.11, 4.4.1.2]

Predicted SEED Role

"Bona fide RidA/YjgF/TdcF/RutC subgroup"

MetaCyc Pathways

• aspartate superpathway (23/25 steps found)
• superpathway of branched chain amino acid biosynthesis (17/17 steps found)
• superpathway of L-lysine, L-threonine and L-methionine biosynthesis I (17/18 steps found)
• superpathway of L-isoleucine biosynthesis I (13/13 steps found)
• superpathway of L-lysine, L-threonine and L-methionine biosynthesis II (13/15 steps found)
• L-isoleucine biosynthesis I (from threonine) (7/7 steps found)
• superpathway of S-adenosyl-L-methionine biosynthesis (8/9 steps found)
• superpathway of L-methionine biosynthesis (transsulfuration) (8/9 steps found)
• superpathway of L-homoserine and L-methionine biosynthesis (7/8 steps found)
• L-serine degradation (3/3 steps found)
• glycine degradation (3/3 steps found)
• L-methionine biosynthesis II (5/6 steps found)
• L-threonine degradation I (5/6 steps found)
• L-methionine biosynthesis I (4/5 steps found)
• superpathway of L-threonine metabolism (13/18 steps found)
• D-serine degradation (2/3 steps found)
• L-cysteine degradation II (2/3 steps found)
• L-methionine degradation II (2/3 steps found)
• L-tryptophan degradation II (via pyruvate) (2/3 steps found)
• superpathway of sulfur amino acid biosynthesis (Saccharomyces cerevisiae) (7/10 steps found)
• L-threonine degradation V (1/2 steps found)
• seleno-amino acid biosynthesis (plants) (3/5 steps found)
• glycine betaine degradation III (4/7 steps found)
• sulfolactate degradation III (1/3 steps found)
• purine nucleobases degradation II (anaerobic) (16/24 steps found)
• superpathway of L-cysteine biosynthesis (fungi) (3/6 steps found)
• felinine and 3-methyl-3-sulfanylbutan-1-ol biosynthesis (2/5 steps found)
• superpathway of L-cysteine biosynthesis (mammalian) (2/5 steps found)
• L-mimosine degradation (4/8 steps found)
• glycine betaine degradation I (4/8 steps found)
• homocysteine and cysteine interconversion (1/4 steps found)
• L-cysteine biosynthesis VI (reverse transsulfuration) (3/7 steps found)
• superpathway of sulfolactate degradation (2/6 steps found)
• glutathione-mediated detoxification I (3/8 steps found)
• hydrogen sulfide biosynthesis II (mammalian) (1/6 steps found)
• glutathione-mediated detoxification II (1/9 steps found)
• superpathway of L-methionine salvage and degradation (6/16 steps found)
• hypoglycin biosynthesis (4/14 steps found)
• superpathway of seleno-compound metabolism (6/19 steps found)
• cyclosporin A biosynthesis (2/15 steps found)

KEGG Metabolic Maps

• Cysteine metabolism
• Glycine, serine and threonine metabolism
• Methionine metabolism
• Nitrogen metabolism
• Propanoate metabolism
• Selenoamino acid metabolism
• Sulfur metabolism
• Tryptophan metabolism
• Tyrosine metabolism
• Valine, leucine and isoleucine biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 3.5.99.7, 4.3.1.17, 4.3.1.19

Use Curated BLAST to search for 3.5.99.10 or 3.5.99.7 or 4.1.99.1 or 4.1.99.2 or 4.3.1.13 or 4.3.1.17 or 4.3.1.18 or 4.3.1.19 or 4.3.3.8 or 4.4.1.1 or 4.4.1.11 or 4.4.1.13 or 4.4.1.15 or 4.4.1.2 or 4.4.1.25 or 4.4.1.28 or 4.4.1.35 or 4.5.1.2

Protein Sequence (128 amino acids)

>MIT1002_00109 Putative reactive intermediate deaminase TdcF (Alteromonas macleodii MIT1002)
MSKSIIQTDKAPAAIGTYSQAVKAGTTVYLSGQIPLVAETMKMVSDDFEAQAVQVFENIK
AVCDAAGGTTNDLVKVNIFLIDLGHFATVNEIMSRYFTKPYPARVAVQVSALPKGAQIEI
DGVMELPE