Protein Info for MIT1002_00108 in Alteromonas macleodii MIT1002

Annotation: Bifunctional (p)ppGpp synthase/hydrolase SpoT

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 703 TIGR00691: RelA/SpoT family protein" amino acids 27 to 696 (670 residues), 844.4 bits, see alignment E=3.3e-258 PF13328: HD_4" amino acids 27 to 175 (149 residues), 180 bits, see alignment E=9.3e-57 PF01966: HD" amino acids 45 to 144 (100 residues), 46.1 bits, see alignment E=2e-15 PF04607: RelA_SpoT" amino acids 235 to 344 (110 residues), 141.4 bits, see alignment E=4.9e-45 PF02824: TGS" amino acids 388 to 447 (60 residues), 71.8 bits, see alignment 1.3e-23 PF19296: RelA_AH" amino acids 474 to 540 (67 residues), 30.2 bits, see alignment E=1.1e-10 PF28438: RelA_RIS" amino acids 555 to 616 (62 residues), 70.3 bits, see alignment 3.1e-23 PF13291: ACT_4" amino acids 620 to 697 (78 residues), 60.3 bits, see alignment E=8.5e-20

Best Hits

Swiss-Prot: 60% identical to SPOT_VIBCH: Guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase (spoT) from Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)

KEGG orthology group: None (inferred from 98% identity to amc:MADE_00155)

Predicted SEED Role

"GTP pyrophosphokinase (EC 2.7.6.5), (p)ppGpp synthetase II / Guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase (EC 3.1.7.2)" in subsystem Stringent Response, (p)ppGpp metabolism (EC 2.7.6.5, EC 3.1.7.2)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.6.5

Use Curated BLAST to search for 2.7.6.5 or 3.1.7.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (703 amino acids)

>MIT1002_00108 Bifunctional (p)ppGpp synthase/hydrolase SpoT (Alteromonas macleodii MIT1002)
MYLFEGLKQKVLEYLPADRVQLVQESFVLAQEAHDGQMRSSGDPYITHPVAVAGILADMH
LDHETIMAALLHDVIEDTHYSKEDLAEAFGDTVAELVEGVSKLDKLAFSTKQEAQAENFR
KMMMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIALETLEIYAPIAHRLGIHDIKN
ELEDLGFQAMYPMRHRALKSAVRQARGNRKEIIENIREELNTRLDAYQIESNVLGREKHL
YSIYRKMKNKELMFNEVMDIYAFRIVVQSVDNCYRALGAMHGLYKPIENRFKDYIAIPRT
NGYQSLHTSLIGPHGIPVEIQIRTQEMDQMADKGVAAHWLYKEPGDNGTTAQLRARKWMQ
SLLELQQSASSSFEFIESVKTDLFPDEIYVFTPDGRIIELPMGATAVDFAYAVHSDVGNT
CVGVRVERRNFSLSKPLENGQTVEIITSPKAKPNANWLNFVVSARARTRIRQYLRKQHSQ
EAVNMGNRLLRHALGSVKLDDIPNEDIERVVAETKHANFDDLLVDIGLGNELSAIVARRL
LGESTTDLSDKKGNVAIRGTEGLLVHYSRCCHPIPDDEIVAVLSPGRGMTIHQIGCNNIR
KLTREEPQRVLPMQWDDNPQGEFKASLRIELFNHQGTLATLTNTISGCDSNIIGLQTEEK
ESNIYFIDLELTTHNRVHLARVMKKIRTMPEVQRVSRHSQSRH