Protein Info for MIT1002_00097 in Alteromonas macleodii MIT1002

Annotation: Phosphomethylpyrimidine synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF13667: ThiC-associated
  • amino acids 23 to 103 (81 residues), 97.2 bits, E=4e-32
• TIGR00190: phosphomethylpyrimidine synthase
  • amino acids 143 to 595 (453 residues), 728.8 bits, E=8.9e-224
• PF01964: ThiC_Rad_SAM
  • amino acids 144 to 591 (448 residues), 624.5 bits, E=8.4e-192

Best Hits

Swiss-Prot: 96% identical to THIC_ALTMD: Phosphomethylpyrimidine synthase (thiC) from Alteromonas mediterranea (strain DSM 17117 / CIP 110805 / LMG 28347 / Deep ecotype)

KEGG orthology group: K03147, thiamine biosynthesis protein ThiC (inferred from 96% identity to amc:MADE_00146)

MetaCyc: 79% identical to phosphomethylpyrimidine synthase (Escherichia coli K-12 substr. MG1655)
PYRIMSYN1-RXN [EC: 4.1.99.17]

Predicted SEED Role

"Hydroxymethylpyrimidine phosphate synthase ThiC (EC 4.1.99.17)" (EC 4.1.99.17)

MetaCyc Pathways

• superpathway of thiamine diphosphate biosynthesis I (9/10 steps found)
• superpathway of thiamine diphosphate biosynthesis II (9/11 steps found)
• 4-amino-2-methyl-5-diphosphomethylpyrimidine biosynthesis I (2/2 steps found)
• superpathway of thiamine diphosphate biosynthesis III (eukaryotes) (3/7 steps found)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.1.99.17

Protein Sequence (636 amino acids)

>MIT1002_00097 Phosphomethylpyrimidine synthase (Alteromonas macleodii MIT1002)
MSNRREQRQQAQQFINELAGEAYPNSTRHYECGSRDDIRVAMRLIHQHDSLVGGTEDNPI
LEPNPPIPVYDTSGPYGDPLENIDVHKGLIPLRQKWIEERSDTIELEGVSSSFAKEREND
IFTEDFRFIRSRKPLKAKAGKNVTQLHYARQGIITPEMEYIAIRENMGRQAIKDAELTQQ
HKGEHFGANLPDTITPEFVRQEVAAGRAIIPCNINHPELEPMIIGRNFLVKINANIGNSA
VTSSIEEEVEKLVWSTRWGADTIMDLSTGRNIHETREWLLRNSPVPLGTVPIYQALEKVN
GVAEDLTWEMFKDTLIEQAEQGVDYFTIHAGVLLEYVHLTAKRVTGIVSRGGSIMAKWCL
SHHQQSFLYEHFHDICEICAKYDVALSLGDGLRPGSVADANDDAQFSELRTLGELTKIAW
EYDVQVMIEGPGHVPMHMIKANMEEQLKHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAM
IGWYGCAMLCYVTPKEHLGLPNKEDVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKAR
FEFRWEDQFNLALDPHTARAYHDETLPQASGKVAHFCSMCGPKFCSMKISQEVRDVAKQA
EQQKFEKEKGMKDMAQAFNQSGAELYHTADSKVELP