Protein Info for MIT1002_00096 in Alteromonas macleodii MIT1002

Annotation: Thiamine-phosphate synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF08543: Phos_pyr_kin
  • amino acids 28 to 262 (235 residues), 165 bits, E=3e-52
• PF00294: PfkB
  • amino acids 137 to 260 (124 residues), 31 bits, E=2.6e-11
• PF02581: TMP-TENI
  • amino acids 344 to 513 (170 residues), 173 bits, E=6.2e-55
• TIGR00693: thiamine-phosphate diphosphorylase
  • amino acids 344 to 525 (182 residues), 170.8 bits, E=1e-54

Predicted SEED Role

"Phosphomethylpyrimidine kinase (EC 2.7.4.7) / Thiamin-phosphate pyrophosphorylase (EC 2.5.1.3)" in subsystem Thiamin biosynthesis (EC 2.5.1.3, EC 2.7.4.7)

MetaCyc Pathways

• superpathway of thiamine diphosphate biosynthesis I (9/10 steps found)
• superpathway of thiamine diphosphate biosynthesis II (9/11 steps found)
• 4-amino-2-methyl-5-diphosphomethylpyrimidine biosynthesis I (2/2 steps found)
• thiamine diphosphate biosynthesis I (E. coli) (2/2 steps found)
• thiamine diphosphate biosynthesis II (Bacillus) (2/2 steps found)
• thiamine diphosphate salvage V (2/3 steps found)
• hydroxymethylpyrimidine salvage (1/2 steps found)
• thiamine diphosphate salvage II (3/5 steps found)
• thiamine diphosphate biosynthesis III (Staphylococcus) (1/3 steps found)
• thiamine diphosphate biosynthesis IV (eukaryotes) (1/3 steps found)
• superpathway of thiamine diphosphate biosynthesis III (eukaryotes) (3/7 steps found)
• thiamine diphosphate salvage IV (yeast) (2/7 steps found)
• 4-amino-2-methyl-5-diphosphomethylpyrimidine biosynthesis II (2/8 steps found)
• thiamine diphosphate formation from pyrithiamine and oxythiamine (yeast) (2/8 steps found)

KEGG Metabolic Maps

• Thiamine metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.5.1.3 or 2.7.4.7

Protein Sequence (533 amino acids)

>MIT1002_00096 Thiamine-phosphate synthase (Alteromonas macleodii MIT1002)
MMAKASISPASNNLTTALPVVWCVGGVDCSGGAGVTRDAITLADLNIHACVLTTQLTVQS
NSIMLSKESMCASALNQQWQVLFEDTPPRAIKIGAIANDEQALLLCARIQKTSNPRPFVV
WDPVLSTSSGGVLSELSESVVDELLNTVDIVTPNIDELAWLTHLPVVDEASLLTAINRLR
GKGAKSVYVKGGHAHWQKNVSDIFVCASHTLRFSQPKYANGNLRGTGCMLASAVAAFIVH
DYCIEDALTLANAYVSEVRNHTLPKQCAAANANAISNVLTAYFARTNGFPAKPESFPLVT
FHQRGATANEKERDKDTLLEASSCKAAHFPALTHTHLGIYPVVDSVQWIARLWPTGVKIM
QLRVKQGSQEDIRQQIKEAIELVKHTDCQLFINDYWELAIELGAYGVHLGQEDIDTANLS
AIRSAGLRLGISTHGFAEIQRVRALNPSYIALGHIFPTNTKDMPSKPQGVARLEKYVQLC
DGIPTVAIGGINLSRIGDVAKTGVNGIAVVSAITQAAHPLKAYQALVEEAGFA