Protein Info for MIT1002_00040 in Alteromonas macleodii MIT1002

Annotation: Error-prone DNA polymerase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1029 TIGR00594: DNA polymerase III, alpha subunit" amino acids 5 to 973 (969 residues), 525.5 bits, see alignment E=1.5e-161 PF02811: PHP" amino acids 8 to 101 (94 residues), 44.2 bits, see alignment E=6.6e-15 PF07733: DNA_pol3_alpha" amino acids 270 to 525 (256 residues), 292.7 bits, see alignment E=7.5e-91 PF17657: DNA_pol3_finger" amino acids 528 to 696 (169 residues), 184.4 bits, see alignment E=2.8e-58 PF14579: HHH_6" amino acids 770 to 855 (86 residues), 53.4 bits, see alignment E=6.1e-18 PF01336: tRNA_anti-codon" amino acids 938 to 1011 (74 residues), 31.3 bits, see alignment 4.1e-11

Best Hits

Swiss-Prot: 63% identical to DNAE2_VIBCB: Error-prone DNA polymerase (dnaE2) from Vibrio campbellii (strain ATCC BAA-1116 / BB120)

KEGG orthology group: K14162, error-prone DNA polymerase [EC: 2.7.7.7] (inferred from 90% identity to amc:MADE_00038)

Predicted SEED Role

"DNA polymerase III alpha subunit (EC 2.7.7.7)" in subsystem DNA-replication (EC 2.7.7.7)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.7.7

Use Curated BLAST to search for 2.7.7.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (1029 amino acids)

>MIT1002_00040 Error-prone DNA polymerase (Alteromonas macleodii MIT1002)
MKYSELFCQSNYSFLCGASSPAELVTTASFLGYDALAITDECSVAGVVRAFDEIKHQGLP
ITLIVGSYFVFDDTLSFVLLCPNKAAYSELCRIITNARRRSDKGEYQLAEWDLRTIRHCK
FIWLPSGKEAKDKHWAEWLQKHPSIDAYIGAQRLLDGRDHHRFAHYNHLQSAYPFPVIAC
TGVLMHHADRLPLQHVLHAINVHTSVDKIGRDALSNAERSLRTVQKIKKLYPEKWIANTN
ALAHAFTFSLEELRYHYPAELVPEGYTPTSYLRERVEAGIKVRFPEGITPDIRQTIEKEL
TLIAEQEYEYFFLTIYDIVQFAKRQRILYQGRGSAANSVVCYCLEITAVDPRQISVLFER
FISKERNEPPDIDVDFEHQRREEVIQYIYQKYGRERTAIAATVICYRFKSAFKDVGKALG
FSESQLDFVVKQINRRDSVIPWKSQLAGLGLNPEEPRVQQLITLTEALLGTPRHLSQHVG
GFVISAGPLYDLVPVENAAMEERTIIQWDKDDIETLGLLKVDVLALGMLSAIRRAFSLIN
KQYPVETSIPFITKLGDDKQVFDMICRADTVGVFQIESRAQMSMLPRLKPRRYYDLVVQI
AIVRPGPIQGDMVHPYLIRRHGNETISYPSKDVEKVLSRTMGVPIFQEQVIQLAMVAAGF
SGGEADQLRRAMASWKKDGRIFEFRDKLIKGMTDKGYDSTFAHNLFEQIKGFAGYGFPES
HSASFAVLAYVSCWLKFYFPVEFYVALLNSWPMGFYSPSQLVQDAKRHHIRVEAPCVNQS
DVEHSLVVQGQEKYIQLGLKLIKGLSEASSQLIVENRSDKGYTDVEQLQQLSLKSNEVEA
LASADALRTISGNRYETRWRIMDTQTHLPLFDTDDFYADKNVTPLGASPTEAENLLEDYA
STGLSVNNHPISLLKKQHKLKDISWSNSLKDKTNKSVVKVLGAVTGRQAPGTAAGVTFLT
LEDDIGNMNVVVWQATARAQQQTFLKAKLLQVNGIIERSKEGVIHIIAGKLIDRTPWLEK
LSLSSRDFH