Protein Info for MIT1002_00018 in Alteromonas macleodii MIT1002

Annotation: Ribosomal RNA small subunit methyltransferase B

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01029: NusB
  • amino acids 17 to 132 (116 residues), 69.4 bits, E=6.3e-23
• TIGR00563: 16S rRNA (cytosine(967)-C(5))-methyltransferase
  • amino acids 18 to 437 (420 residues), 421.8 bits, E=1.9e-130
• PF01189: Methyltr_RsmB-F
  • amino acids 244 to 434 (191 residues), 230.1 bits, E=3.2e-72

Best Hits

KEGG orthology group: K03500, ribosomal RNA small subunit methyltransferase B [EC: 2.1.1.-] (inferred from 91% identity to amc:MADE_00020)

Predicted SEED Role

"Ribosomal RNA small subunit methyltransferase B (EC 2.1.1.-)" in subsystem Conserved gene cluster associated with Met-tRNA formyltransferase (EC 2.1.1.-)

KEGG Metabolic Maps

• Alkaloid biosynthesis I
• Anthocyanin biosynthesis
• Benzoxazinone biosynthesis
• Biosynthesis of alkaloids derived from shikimate pathway
• Carotenoid biosynthesis - General
• Flavonoid biosynthesis
• Histidine metabolism
• Insect hormone biosynthesis
• Naphthalene and anthracene degradation
• Phenylpropanoid biosynthesis
• Porphyrin and chlorophyll metabolism
• Tryptophan metabolism
• Tyrosine metabolism
• Ubiquinone and menaquinone biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 2.1.1.-

Use Curated BLAST to search for 2.1.1.-

Protein Sequence (439 amino acids)

>MIT1002_00018 Ribosomal RNA small subunit methyltransferase B (Alteromonas macleodii MIT1002)
MKPLPLPKQKNLRADCAWVIYQILEQGKSSRECLERVQRRHNAKDNAWIQEMSLGVMRRL
PLLQHWLRSLLDRPLKGNKKVIEHLILLGLYQLNFSRVSNHAAVGETVAAASYLNAAGLK
GLVNAVLRNFQRENLADKLVDDAIINSGLPKWLFKAIENAYGDDAEQVRTETNAMAPIWL
RVNKLQTSLAAFTQALDKAGVEYTLSENHADAVILTKREDITALPGFEKGHFAVQDGAAQ
LAAHYLKPQANERILDCCAAPGGKTGHIVESAPDTQYVLALDADATRLKRVEENMTRLKH
TVDIKQGDAANPDTWWDGKPFDRILLDAPCSATGVIRRHPDIRWLRKANDIDNLAQLQRR
ILETLWGLLKPGGTLLYATCSILPKENMAQIQQFLADTPDASLSPIVKTETVDKPGRQIT
PGEQQMDGFYYARLVKSAD