Protein Info for MCAODC_28015 in Escherichia coli ECRC101
Name: mhpA
Annotation: bifunctional 3-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid hydroxylase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 100% identical to MHPA_ECO5E: 3-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid hydroxylase (mhpA) from Escherichia coli O157:H7 (strain EC4115 / EHEC)
KEGG orthology group: K05712, 3-(3-hydroxy-phenyl)propionate hydroxylase [EC: 1.14.13.-] (inferred from 100% identity to ece:Z0445)MetaCyc: 99% identical to 3-(3-hydroxyphenyl)propanoate hydroxylase (Escherichia coli K-12 substr. MG1655)
MHPHYDROXY-RXN [EC: 1.14.13.127]; 1.14.13.127 [EC: 1.14.13.127]
Predicted SEED Role
"3-(3-hydroxy-phenyl)propionate hydroxylase (EC 1.14.13.-)" in subsystem Cinnamic Acid Degradation (EC 1.14.13.-)
MetaCyc Pathways
- 3-phenylpropanoate and 3-(3-hydroxyphenyl)propanoate degradation (8/8 steps found)
- 3-phenylpropanoate and 3-(3-hydroxyphenyl)propanoate degradation to 2-hydroxypentadienoate (5/5 steps found)
- cinnamate and 3-hydroxycinnamate degradation to 2-hydroxypentadienoate (5/5 steps found)
KEGG Metabolic Maps
- 1,4-Dichlorobenzene degradation
- 1- and 2-Methylnaphthalene degradation
- Alkaloid biosynthesis I
- Benzoate degradation via CoA ligation
- Biosynthesis of alkaloids derived from shikimate pathway
- Biosynthesis of phenylpropanoids
- Biosynthesis of plant hormones
- Biosynthesis of terpenoids and steroids
- Bisphenol A degradation
- Brassinosteroid biosynthesis
- Carotenoid biosynthesis - General
- Cyanoamino acid metabolism
- Flavonoid biosynthesis
- Histidine metabolism
- Isoflavonoid biosynthesis
- Limonene and pinene degradation
- Methane metabolism
- Naphthalene and anthracene degradation
- Phenylalanine metabolism
- Phenylpropanoid biosynthesis
- Styrene degradation
- Toluene and xylene degradation
- Tryptophan metabolism
- Ubiquinone and menaquinone biosynthesis
- gamma-Hexachlorocyclohexane degradation
Isozymes
Compare fitness of predicted isozymes for: 1.14.13.-
Use Curated BLAST to search for 1.14.13.- or 1.14.13.127
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (554 amino acids)
>MCAODC_28015 bifunctional 3-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid hydroxylase (Escherichia coli ECRC101) MAIQHPDIQPAVNHSVQVAIAGAGPVGLMMANYLGQMGIDVLVVEKLDKLIDYPRAIGID DEALRTMQSVGLVDNVLPHTTPWHAMRFLTPKGRCFADIQPMTDEFGWPRRNAFIQPQVD AVMLEGVSRFPNVRCLFSRELEAFSQQDDEVTLHLKTAEGLREIVKAQWLVACDGGASFV RRTLNVPFEGKTAPNQWIVVDIANDPLSTPHIYLCCDPVRPYVSAALPHAVRRFEFMVMP GETEEQLREPQNMRKLLSKVLPNPDNVELIRQRVYTHNARLAQRFRIDRVLLAGDAAHIM PVWQGQGYNSGMRDAFNLAWKLALVIQGKARDALLDTYQQERRDHAKAMIDLSVTAGNVL APPKRWQGTLRDGVSWLLNYLPPVKRYFLEMRFKPMPQYYGGALVREGEAKHSPVGKMFI QPKVTLENGDVTLLDNAIGANFAVIGWGCNPLWGMSDEQIQQWRALSTRFIQVVPEVQIH TAQDNHDGVLRVGDTQGRLRSWFAQHNASLVVMRPDRFVAATAIPQTLGKTLNKLASVMT LTRPDADVSVEKVA