Protein Info for MCAODC_27835 in Escherichia coli ECRC101

Name: proC
Annotation: pyrroline-5-carboxylate reductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF03446: NAD_binding_2
  • amino acids 4 to 96 (93 residues), 35.8 bits, E=1.2e-12
• PF03807: F420_oxidored
  • amino acids 4 to 99 (96 residues), 86.8 bits, E=1.8e-28
• TIGR00112: pyrroline-5-carboxylate reductase
  • amino acids 5 to 266 (262 residues), 309.8 bits, E=8.8e-97
• PF14748: P5CR_dimer
  • amino acids 162 to 266 (105 residues), 139.8 bits, E=5.4e-45

Best Hits

Swiss-Prot: 100% identical to P5CR_ECOLI: Pyrroline-5-carboxylate reductase (proC) from Escherichia coli (strain K12)

KEGG orthology group: K00286, pyrroline-5-carboxylate reductase [EC: 1.5.1.2] (inferred from 100% identity to eco:b0386)

MetaCyc: 100% identical to pyrroline-5-carboxylate reductase (Escherichia coli K-12 substr. MG1655)
Pyrroline-5-carboxylate reductase. [EC: 1.5.1.2]

Predicted SEED Role

"Pyrroline-5-carboxylate reductase (EC 1.5.1.2)" in subsystem Proline Synthesis (EC 1.5.1.2)

MetaCyc Pathways

• L-proline biosynthesis I (from L-glutamate) (4/4 steps found)
• L-proline biosynthesis III (from L-ornithine) (2/3 steps found)
• L-arginine degradation XIII (reductive Stickland reaction) (3/5 steps found)
• L-arginine degradation VI (arginase 2 pathway) (2/4 steps found)

KEGG Metabolic Maps

• Arginine and proline metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.5.1.2

Protein Sequence (269 amino acids)

>MCAODC_27835 pyrroline-5-carboxylate reductase (Escherichia coli ECRC101)
MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAALHDKFGINAAESAQEV
AQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTLDQLARALGHDRKIIRAMP
NTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVIAEPMIHPVVGVSGSSPAYVF
MFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLETGEHPGALKDMVCSPGGTTIEAV
RVLEEKGFRAAVIEAMTKCMEKSEKLSKS