Protein Info for MCAODC_26900 in Escherichia coli ECRC101

Name: cusA
Annotation: Cu(+)/Ag(+) efflux RND transporter permease subunit CusA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1045 signal peptide" amino acids 12 to 12 (1 residues), see Phobius details transmembrane" amino acids 13 to 31 (19 residues), see Phobius details amino acids 337 to 356 (20 residues), see Phobius details amino acids 362 to 382 (21 residues), see Phobius details amino acids 388 to 409 (22 residues), see Phobius details amino acids 441 to 463 (23 residues), see Phobius details amino acids 482 to 503 (22 residues), see Phobius details amino acids 530 to 552 (23 residues), see Phobius details amino acids 872 to 889 (18 residues), see Phobius details amino acids 896 to 916 (21 residues), see Phobius details amino acids 923 to 946 (24 residues), see Phobius details amino acids 983 to 1002 (20 residues), see Phobius details amino acids 1011 to 1036 (26 residues), see Phobius details TIGR00914: heavy metal efflux pump, CzcA family" amino acids 1 to 1045 (1045 residues), 1659.6 bits, see alignment E=0 PF00873: ACR_tran" amino acids 6 to 1035 (1030 residues), 787.6 bits, see alignment E=8.4e-241

Best Hits

Swiss-Prot: 100% identical to CUSA_ECO57: Cation efflux system protein CusA (cusA) from Escherichia coli O157:H7

KEGG orthology group: K07787, Cu(I)/Ag(I) efflux system membrane protein CusA (inferred from 68% identity to aex:Astex_3842)

MetaCyc: 99% identical to copper/silver export system RND permease (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-90; TRANS-RXN0-280

Predicted SEED Role

"Cobalt-zinc-cadmium resistance protein CzcA; Cation efflux system protein CusA"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (1045 amino acids)

>MCAODC_26900 Cu(+)/Ag(+) efflux RND transporter permease subunit CusA (Escherichia coli ECRC101)
MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSDVQVIIKTSYPGQAPQI
VENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTDPYWARSRVLEYLNQVQGK
LPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQDWFLKYELKTIPDVAEVASV
GGVVKEYQVVIDPQRLAQYGISLAEVKSALDASNQEAGGSSIELAEAEYMVRASGYLQTL
DDFNHIVLKASENGVPVYLRDVAKIQVGPEMRRGIAELNGEVVGGVVILRSGKNAREVIA
AVKDKLETLKSSLPEGVEIVTTYDRSQLIDRAIDNLSGKLLEEFIVVAVVCALFLWHVRS
ALVAIISLPLGLCIAFIVMHFQGLNANIMSLGGIAIAVGAMVDAAIVMIENAHKRLEEWQ
HQHPDATLDNKTRWQVITNASVEVGPALFISLLIITLSFIPIFTLEGQEGRLFGPLAFTK
TYAMAGAALLAIVVIPILMGYWIRGKIPPESSNPLNRFLIRVYHPLLLKVLHWPKTTLLV
AALSVLTVLWPLNKVGGEFLPQINEGDLLYMPSTLPGISAAEAASMLQKTDKLIMSVPEV
ARVFGKTGKAETATDSAPLEMVETTIQLKPQEQWRPGMTMDKIIEELDNTVRLPGLANLW
VPPIRNRIDMLSTGIKSPIGIKVSGTVLADIDAMAEQIEEVARTVPGVASALAERLEGGR
YINVEINREKAARYGMTVADVQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQA
LRQLPILTPMKQQITLADVADVKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQK
AIAEKVQLKPGTSVAFSGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIIS
SVPFALVGGIWLLWWMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAEPSLNNPQ
TFSEQKLDEALHHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMIT
APLLSLFIIPAAYKLMWLHRHRVRK