Protein Info for MCAODC_26090 in Escherichia coli ECRC101
Name: glmE
Annotation: methylaspartate mutase subunit E
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 100% identical to GLME_ECO57: Glutamate mutase epsilon subunit (glmE) from Escherichia coli O157:H7
KEGG orthology group: None (inferred from 100% identity to etw:ECSP_0780)MetaCyc: 58% identical to GlmE (Clostridium cochlearium)
Predicted SEED Role
"Methylaspartate mutase, E subunit (EC 5.4.99.1)" (EC 5.4.99.1)
MetaCyc Pathways
- L-isoleucine biosynthesis III (5/7 steps found)
- L-glutamate degradation VI (to pyruvate) (2/4 steps found)
- L-glutamate degradation VIII (to propanoate) (7/11 steps found)
- L-glutamate degradation VII (to butanoate) (7/12 steps found)
- methylaspartate cycle (11/19 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 5.4.99.1
Use Curated BLAST to search for 5.4.99.1
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (481 amino acids)
>MCAODC_26090 methylaspartate mutase subunit E (Escherichia coli ECRC101) MELRNKKLTHDEFMTERQQVLKTWETGKDVENFEDGVKYQQTIPEHKRFSLALLKADKEG KTLSQPRAGVALMDEHIELLKTLQEECDLLPSTIDAYTRLNRYEEAAVGIKKSIEAGTSK LNGLPVVNHGVAACRRLTETLQKPLQIRHGTPDARLLAEISMASGFTSYEGGGISYNIPY AKRVTLEKSIRDWQYCDRLMGMYEEHGIRINREPFGPLTGTLIPPFISHSIAIIEGLLAL EQGVKSITVGYGQVGSLTQDVAAIQSLRELAHEYFQSYGYTDYELSTVFHQWMGGFPEDE SKAFAIISWGAAVAGMSGATKVITKSPHEAWGIPTAAANIQGLKASRQMLNMVNEQKFPP CPAVELEIELIKSEVRAVLNKVFELGNGDIARGTVLAFEAGVLDVPFAPAACNAGKILPV RDNTGAIRVLEAGAVPLPKDILDLHHDYVAERARCEGRQPTFQMVVDDINAVSHSKLIGR P