Protein Info for MCAODC_25610 in Escherichia coli ECRC101

Name: bioF
Annotation: 8-amino-7-oxononanoate synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 384 transmembrane" amino acids 102 to 121 (20 residues), see Phobius details TIGR00858: 8-amino-7-oxononanoate synthase" amino acids 20 to 381 (362 residues), 495.7 bits, see alignment E=3.1e-153 PF00155: Aminotran_1_2" amino acids 40 to 380 (341 residues), 238.9 bits, see alignment E=5.2e-75

Best Hits

Swiss-Prot: 100% identical to BIOF_ECO57: 8-amino-7-oxononanoate synthase (bioF) from Escherichia coli O157:H7

KEGG orthology group: K00652, 8-amino-7-oxononanoate synthase [EC: 2.3.1.47] (inferred from 98% identity to eco:b0776)

MetaCyc: 98% identical to 8-amino-7-oxononanoate synthase (Escherichia coli K-12 substr. MG1655)
8-amino-7-oxononanoate synthase. [EC: 2.3.1.47]; 2.3.1.47 [EC: 2.3.1.47]

Predicted SEED Role

"8-amino-7-oxononanoate synthase (EC 2.3.1.47)" in subsystem Biotin biosynthesis (EC 2.3.1.47)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.3.1.47

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (384 amino acids)

>MCAODC_25610 8-amino-7-oxononanoate synthase (Escherichia coli ECRC101)
MSWQEKINAALDARRAADALRRRYPVAQGAGRWLVADDRQYLNFSSNDYLGLSHHPQIIR
AWKQGAEQFGVGSGGSGHVSGYSVAHQALEEELAEWLGYSRALLFISGFAANQAVIAAMM
AKEDRIVADRLSHASLLEAASLSPSQLRRFVHNDVTHLARLLASPCPGQQLVVTEGVFSM
DGDSAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRGTCWLQKVKPELLVVTFGKGFGV
SGAAVLCSSTVADYLLQFARHLIYSTSMPPAQAQALRASLAVIRSDEGDARREKLAALIT
RFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQLAEKLRQQGCWVTAIRPPTVPSGTARL
RLTLTAAHEMQDIDRLLEVLHGNG