Protein Info for MCAODC_25025 in Escherichia coli ECRC101

Name: macA
Annotation: macrolide transporter subunit MacA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 371 transmembrane" amino acids 12 to 29 (18 residues), see Phobius details TIGR01730: efflux transporter, RND family, MFP subunit" amino acids 40 to 362 (323 residues), 188.9 bits, see alignment E=5.5e-60 PF16576: HlyD_D23" amino acids 47 to 294 (248 residues), 60.2 bits, see alignment E=2.8e-20 PF13533: Biotin_lipoyl_2" amino acids 62 to 108 (47 residues), 33.3 bits, see alignment 5e-12 PF13437: HlyD_3" amino acids 186 to 284 (99 residues), 53.4 bits, see alignment E=5.6e-18

Best Hits

Swiss-Prot: 100% identical to MACA_SHIFL: Macrolide export protein MacA (macA) from Shigella flexneri

KEGG orthology group: K13888, macrolide-specific efflux protein MacA (inferred from 99% identity to eco:b0878)

MetaCyc: 99% identical to ABC-type tripartite efflux pump membrane fusion protein (Escherichia coli K-12 substr. MG1655)
7.6.2.-; 7.6.2.-; 7.6.2.-

Predicted SEED Role

"Macrolide-specific efflux protein MacA" in subsystem Multidrug Resistance Efflux Pumps

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (371 amino acids)

>MCAODC_25025 macrolide transporter subunit MacA (Escherichia coli ECRC101)
MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPGDLQQSVLATGKLDALR
KVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIKEVEATLMELRAQRQQAEA
ELKLARVTYSRQQRLAQTQAVSLQDLDTAATEMAVKQAQIGTIDAQIKRNQASLDTAKTN
LDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLADMSTMLVKAQVSEADVIHLKPG
QKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYYARFEVPNPNGLLRLDMTAQVHIQL
TDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETREREVTIGARNDTDVEIVKGLEAGDEV
VIGEAKPGAAQ