Protein Info for MCAODC_24670 in Escherichia coli ECRC101

Name: rlmKL
Annotation: bifunctional 23S rRNA (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA (guanine(2445)-N(2))-methyltransferase RlmL

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 702 transmembrane" amino acids 459 to 479 (21 residues), see Phobius details PF02926: THUMP" amino acids 21 to 153 (133 residues), 81.5 bits, see alignment E=3.4e-26 PF01170: UPF0020" amino acids 162 to 375 (214 residues), 215.4 bits, see alignment E=3.3e-67 PF10672: Methyltrans_SAM" amino acids 475 to 658 (184 residues), 71.1 bits, see alignment E=4.6e-23 PF03602: Cons_hypoth95" amino acids 532 to 637 (106 residues), 38.8 bits, see alignment E=4e-13

Best Hits

Swiss-Prot: 100% identical to RLMKL_ECO57: Ribosomal RNA large subunit methyltransferase K/L (rlmL) from Escherichia coli O157:H7

KEGG orthology group: K12297, ribosomal RNA large subunit methyltransferase L [EC: 2.1.1.173] (inferred from 100% identity to ecj:JW0931)

MetaCyc: 100% identical to fused 23S rRNA m2G2445 methyltransferase and 23S rRNA m7G2069 methyltransferase (Escherichia coli K-12 substr. MG1655)
RXN0-6950 [EC: 2.1.1.264]; RXN-11574 [EC: 2.1.1.264, 2.1.1.173]

Predicted SEED Role

"23S rRNA (guanine-N-2-) -methyltransferase rlmL EC 2.1.1.-)"

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.1.1.173 or 2.1.1.264

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (702 amino acids)

>MCAODC_24670 bifunctional 23S rRNA (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA (guanine(2445)-N(2))-methyltransferase RlmL (Escherichia coli ECRC101)
MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLVYQSLMWSRLASRIMLP
LGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTIRNSQYGAMKVKDAIVDAF
TRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLHLRGYRDRAGIAPIKETLAAA
IVMRSGWQPGTPLLDPMCGSGTLLIEAAMLATDRAPGLHRGRWGFSGWAQHDEAIWQEVK
AEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNARLAGIGELITFEVKDVAQLTNPLPK
EPYGTVLSNPPYGERLDSEPALIALHSLLGRIMKNQFGGWNLSLFSASPDLLSCLQLRAD
KQYKAKNGPLDCVQKNYHVAESTPDSKPAMVAEDYANRLRKNLKKFEKWARQEGIECYRL
YDADLPEYNVAVDRYADWVVVQEYAPPKTIDAHKARQRLFDIIAATISVLGIAPNKLVLK
TRERQKGKNQYQKLGEKGEFLEVTEYNAHLWVNLTDYLDTGLFLDHRIARRMLGQMSKGK
DFLNLFSYTGSATVHAGLGGARSTTTVDMSRTYLEWAERNLRLNGLTGRAHRLIQADCLA
WLREANEQFDLIFIDPPTFSNSKRMEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRG
FRMDLDGLAKLGLKAQEITQKTLSQDFARNRQIHNCWLITAA