Protein Info for MCAODC_24170 in Escherichia coli ECRC101

Annotation: hydrogenase expression/formation protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 285 PF04809: HupH_C" amino acids 51 to 140 (90 residues), 31.1 bits, see alignment E=8.7e-12 amino acids 164 to 279 (116 residues), 114.6 bits, see alignment E=1.2e-37

Best Hits

Swiss-Prot: 99% identical to HYAF_ECOLI: Hydrogenase-1 operon protein HyaF (hyaF) from Escherichia coli (strain K12)

KEGG orthology group: K03618, hydrogenase-1 operon protein HyaF (inferred from 99% identity to eco:b0977)

Predicted SEED Role

"Hydrogenase maturation factor HoxQ"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (285 amino acids)

>MCAODC_24170 hydrogenase expression/formation protein (Escherichia coli ECRC101)
MSETFFHLLGPRTQPNDDSFSMNPLPITCQVNDEPSMAALEQCAHSPQVIALLNELQHQL
SQRQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQADDSESEIQEAIFCGLWRV
RRRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPPIDGLMNGLPLAHELLAHVRN
PDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIGYGESYINATGLRHVWHLRCTDT
LKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVCQWLAEAAPT