Protein Info for MCAODC_20680 in Escherichia coli ECRC101

Name: mASE1
Annotation: diguanylate cyclase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 935 transmembrane" amino acids 20 to 37 (18 residues), see Phobius details amino acids 42 to 58 (17 residues), see Phobius details amino acids 65 to 87 (23 residues), see Phobius details amino acids 100 to 120 (21 residues), see Phobius details PF05231: MASE1" amino acids 1 to 124 (124 residues), 36.3 bits, see alignment E=1.4e-12 TIGR00229: PAS domain S-box protein" amino acids 129 to 252 (124 residues), 118.7 bits, see alignment E=1.7e-38 amino acids 383 to 506 (124 residues), 46.2 bits, see alignment E=4.7e-16 PF00989: PAS" amino acids 133 to 244 (112 residues), 36.3 bits, see alignment E=2e-12 amino acids 390 to 498 (109 residues), 31.8 bits, see alignment E=4.8e-11 PF13426: PAS_9" amino acids 144 to 246 (103 residues), 42.2 bits, see alignment E=3.4e-14 amino acids 396 to 500 (105 residues), 30.6 bits, see alignment E=1.4e-10 PF08448: PAS_4" amino acids 144 to 249 (106 residues), 28 bits, see alignment E=8.7e-10 amino acids 391 to 502 (112 residues), 40.4 bits, see alignment E=1.2e-13 PF08447: PAS_3" amino acids 158 to 238 (81 residues), 39.7 bits, see alignment E=2e-13 amino acids 282 to 367 (86 residues), 38.8 bits, see alignment E=3.7e-13 TIGR00254: diguanylate cyclase (GGDEF) domain" amino acids 510 to 672 (163 residues), 127 bits, see alignment E=5.8e-41 PF00990: GGDEF" amino acids 512 to 668 (157 residues), 155 bits, see alignment E=5.8e-49 PF00563: EAL" amino acids 728 to 918 (191 residues), 70 bits, see alignment E=8.7e-23

Best Hits

Swiss-Prot: 99% identical to DGCE_ECOLI: Probable diguanylate cyclase DgcE (dgcE) from Escherichia coli (strain K12)

KEGG orthology group: None (inferred from 100% identity to ece:Z3235m)

Predicted SEED Role

"FIG00639031: hypothetical protein"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (935 amino acids)

>MCAODC_20680 diguanylate cyclase (Escherichia coli ECRC101)
VPLGLLFKPHYLLRHRNPRLLFESLLTLAITLTLSWLSMLYLPWPFTFIIVLLMWSAVRL
PRMEAFLIFLTTVMMVSLMMAADPSLLATPRTYLMSHMPWLPFLLILLPANIMTMVMYAF
RAERKHISESETRFRNAMEYSAIGMALVGTEGQWLQSNKALCQFLGYSQEELRGLTFQQL
TWPEDLNKDLQQVEKLISGEINTYSMEKRYYNRNGDVVWALLAVSLVRHTDGTPLYFIAQ
IEDINELKRTEQVNQQLMERITLANEAGGIGIWEWELKPNIFSWDKRMFELYEIPPHIKP
NWQVWYECVLPEDRQHAEKVIRDSLQSRSPFKLEFRIAVKDGIRHIRALANRVLNKEGEV
ERLLGINMDMTEVKQLNEALFQEKERLHITLDSIGEAVVCIDMAMKITFMNPVAEKMSGW
TQEEALGVPLLTVLHITFGDNGPLMENIYSADTSRSALEQDVVLHCRSGGSYDVHYSITP
LSTLDGSNIGSVLVIQDVTESRKMLRQLSYSASHDALTHLANRASFEKQLRILLQTVNST
HQRHALVFIDLDRFKAVNDSAGHAAGDALLRELASLMLSMLRSSDVLARLGGDEFGLLLP
DCNVESARFIATRIISAVNDYHFIWEGRVHRVGASAGITLIDDNNHQAAEVMSQADIACY
ASKNGGRGRVTVYEPQQAAAHSERAAMSLDEQWRMIKENQLMMLAHGVASPRIPEARNLW
LISLKLWSCEGEIIDEQTFRRSFSDPALSHALDRRVFHEFLQQAAKAVASKGISIALPLS
VAGLSSATLVNDLLEQLENSPLPPRLLHLIIPAEAIFDHAESVQKLRLGGCRIVFSQVGR
DLQIFNSLKANMADYLLLDGELCANVQGNLMDEMLITIIQGHAQRLGMKTIAGPVVLPLV
MDTLSGIGVDLIYGEVIADAQPLDLLMNSSYFAIN