Protein Info for MCAODC_19195 in Escherichia coli ECRC101

Name: torZ
Annotation: trimethylamine N-oxide reductase TorZ

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 809 signal peptide" amino acids 1 to 32 (32 residues), see Phobius details TIGR01409: Tat (twin-arginine translocation) pathway signal sequence" amino acids 3 to 31 (29 residues), 25.8 bits, see alignment (E = 9.4e-10) PF18364: Molybdopterin_N" amino acids 40 to 80 (41 residues), 43 bits, see alignment 5.6e-15 TIGR00509: molybdopterin guanine dinucleotide-containing S/N-oxide reductases" amino acids 41 to 808 (768 residues), 1263.1 bits, see alignment E=0 PF00384: Molybdopterin" amino acids 84 to 550 (467 residues), 291.1 bits, see alignment E=1.7e-90 PF01568: Molydop_binding" amino acids 670 to 779 (110 residues), 84.6 bits, see alignment E=7.4e-28

Best Hits

Swiss-Prot: 100% identical to TORZ_ECO57: Trimethylamine-N-oxide reductase 2 (torZ) from Escherichia coli O157:H7

KEGG orthology group: K07812, trimethylamine-N-oxide reductase (cytochrome c) 2 [EC: 1.7.2.3] (inferred from 100% identity to ece:Z2925)

MetaCyc: 99% identical to trimethylamine N-oxide reductase 2 (Escherichia coli K-12 substr. MG1655)
Trimethylamine-N-oxide reductase (cytochrome c). [EC: 1.7.2.3]

Predicted SEED Role

"Trimethylamine-N-oxide reductase (EC 1.6.6.9)" in subsystem trimethylamine N-oxide (TMAO) reductase (EC 1.6.6.9)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.6.6.9, 1.7.2.3

Use Curated BLAST to search for 1.6.6.9 or 1.7.2.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (809 amino acids)

>MCAODC_19195 trimethylamine N-oxide reductase TorZ (Escherichia coli ECRC101)
MTLTRREFIKHSGIAAGALVVTSAAPLPAWAEEKGGKILTAGRWGAMNVEVKDGKIVSST
GALAKTIPNSLQSTAADQVHTTARIQHPMVRKSYLDNPLQPAKGRGEDTYVQVSWEQALK
LIHEQHERIRKANGPSAIFAGSYGWRSSGVLHKAQTLLQRYMNLAGGYSGHSGDYSTGAA
QVIMPHVVGSVEVYEQQTSWPLILENSQVVVLWGMNPLNTLKIAWSSTDEQGLEYFHQLK
KSGKPVIAIDPIRSETIEFFGYNATWIAPNMGTDVALMLGIAHTLMTQGKHDKVFLEKYT
TGYPQFEEYLTGKSDNTPKSAAWTAEITGVPEAQIVKLAELMAANRTMLMAGWGIQRQQY
GEQKHWMLVTLAAMLGQIGTPGGGFGFSYHYSNGGNPTRVGGVLPEMSAAIAGQASEAAD
DGGMTAIPVARIVDALENPGGKYQHNGKEQTYPNIKMIWWAGGGNFTHHQDTNRLIKAWQ
KPEMIVVSECYWTAAAKHADIVLPITTSFERNDLTMTGDYSNQHIVPMKQAVAPQFEARN
DFDVFAELAELLKPGGKEIYTEGKDEMAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKL
IEMRRSEKNEQYVRYGDFRADPVKNALGTPSGKIEIYSKTLEKFGYKDCPAHPTWLAPDE
WKGTADEKQLQLLTAHPAHRLHSQLNYAQLRKKYAVADREPITIHTEDAARFGIANGDLV
RVWNKRGQILTGAVVTDGIKKGVVCVHEGAWPDLENGLCKNGSANVLTADIPSSQLANAC
AGNSALVYIEKYTGNAPKLTAFDQPAVQA