Protein Info for MCAODC_14705 in Escherichia coli ECRC101

Name: bglX
Annotation: beta-glucosidase BglX

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 755 PF00933: Glyco_hydro_3" amino acids 36 to 342 (307 residues), 364.9 bits, see alignment E=6.6e-113 PF01915: Glyco_hydro_3_C" amino acids 385 to 638 (254 residues), 206.5 bits, see alignment E=6.5e-65 PF14310: Fn3-like" amino acids 676 to 744 (69 residues), 88.1 bits, see alignment E=5e-29

Best Hits

Swiss-Prot: 100% identical to BGLX_ECOLI: Periplasmic beta-glucosidase (bglX) from Escherichia coli (strain K12)

KEGG orthology group: K05349, beta-glucosidase [EC: 3.2.1.21] (inferred from 100% identity to ect:ECIAI39_0864)

MetaCyc: 100% identical to beta-D-glucoside glucohydrolase, periplasmic (Escherichia coli K-12 substr. MG1655)
Beta-glucosidase. [EC: 3.2.1.21]

Predicted SEED Role

"Periplasmic beta-glucosidase (EC 3.2.1.21)" in subsystem Beta-Glucoside Metabolism (EC 3.2.1.21)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.2.1.21

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (755 amino acids)

>MCAODC_14705 beta-glucosidase BglX (Escherichia coli ECRC101)
VSLALQPALADDLFGNHPLTPEARDAFVTELLKKMTVDEKIGQLRLISVGPDNPKEAIRE
MIKDGQVGAIFNTVTRQDIRAMQDQVMELSRLKIPLFFAYDVLHGQRTVFPISLGLASSF
NLDAVKTVGRVSAYEAADDGLNMTWAPMVDVSRDPRWGRASEGFGEDTYLTSIMGKTMVE
AMQGKSPADRYSVMTSVKHFAAYGAVEGGKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGA
VMVALNSLNGTPATSDSWLLKDVLRDQWGFKGITVSDHGAIKELIKHGTAADPEDAVRVA
LKSGINMSMSDEYYSKYLPGLIKSGKVTMAELDDAARHVLNVKYDMGLFNDPYSHLGPKE
SDPVDTNAESRLHRKEAREVARESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSW
SAAGVADQSVTVLTGIKNAVGENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQE
MIDEAVQTAKQSDVVVAVVGEAQGMAHEASSRTDITILQSQRDLIAALKATGKPLVLVLM
NGRPLALVKEDQQADAILETWFAGTEGGNAIADVLFGDYNPSGKLPMSFPRSVGQIPVYY
SHLNTGRPYNADKPNKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVT
ASVQVTNTGKREGATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEAL
KFWNQQMKYDAEPGKFNVFIGTDSARVKKGEFELL