Protein Info for MCAODC_14255 in Escherichia coli ECRC101

Name: apbE
Annotation: FAD:protein FMN transferase ApbE

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 351 signal peptide" amino acids 1 to 23 (23 residues), see Phobius details PF02424: ApbE" amino acids 42 to 325 (284 residues), 277.2 bits, see alignment E=7.6e-87

Best Hits

Swiss-Prot: 100% identical to APBE_ECO57: FAD:protein FMN transferase (apbE) from Escherichia coli O157:H7

KEGG orthology group: K03734, thiamine biosynthesis lipoprotein (inferred from 100% identity to eco:b2214)

MetaCyc: 100% identical to FAD:protein FMN transferase (Escherichia coli K-12 substr. MG1655)
RXN-14461 [EC: 2.7.1.180]

Predicted SEED Role

"Thiamin biosynthesis lipoprotein ApbE"

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.1.180

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (351 amino acids)

>MCAODC_14255 FAD:protein FMN transferase ApbE (Escherichia coli ECRC101)
MEISFTRVALLAAALFFVGCDQKPQPAKTHATEVTVLEGKTMGTFWRASIPGIDAKRSAE
LKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEAMADIVTTSLRIGAKTDGA
MDITVGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQHLTVINQSHQQYLQKDLPDLY
VDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSRGMNGEGLPWRVAIQKPTDKENA
VQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQTGRPIEHNLVSVTVIAPTALEADA
WDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSFKTWMSPQFKSFLVSEKN