Protein Info for MCAODC_06080 in Escherichia coli ECRC101

Name: ligB
Annotation: NAD-dependent DNA ligase LigB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 560 signal peptide" amino acids 1 to 25 (25 residues), see Phobius details PF01653: DNA_ligase_aden" amino acids 115 to 283 (169 residues), 79.6 bits, see alignment E=2.4e-26 PF03120: DNA_ligase_OB" amino acids 312 to 386 (75 residues), 74.7 bits, see alignment E=4.3e-25

Best Hits

Swiss-Prot: 100% identical to LIGB_ECO57: DNA ligase B (ligB) from Escherichia coli O157:H7

KEGG orthology group: K01972, DNA ligase (NAD+) [EC: 6.5.1.2] (inferred from 100% identity to ecs:ECs4522)

MetaCyc: 98% identical to DNA ligase B (Escherichia coli K-12 substr. MG1655)
DNA ligase (NAD(+)). [EC: 6.5.1.2, 6.5.1.6]

Predicted SEED Role

"DNA ligase (EC 6.5.1.2), LigB" (EC 6.5.1.2)

Isozymes

Compare fitness of predicted isozymes for: 6.5.1.2

Use Curated BLAST to search for 6.5.1.2 or 6.5.1.6

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (560 amino acids)

>MCAODC_06080 NAD-dependent DNA ligase LigB (Escherichia coli ECRC101)
MKVWMAILISILCWQSSVWAVCPAWSPARAQEEIFRLQQQIKQWDDDYWKEGKSEVEDGV
YDQLSARLTQWQRCFVSEPRDVMMPPLNGAVMHPVAHTGVRKMADKNALSLWMRERSDLW
VQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVSLISAVPQTVSGPLANSTLQGE
IFLQREGHIQQQMGGINARAKVAGLMMRQDDSDTLNSLGVFVWAWPDGPQLMTDRLKELA
TAGFTLTQRYTRAVKNADEVARVRNEWWKAKLPFVTDGVVVRGAKEPESRHWLPGQAEWL
VAWKYQPVAQVAKVKAIQFAVGKSGKISVVASLAPVMLDDKKVQRVNIGSVRRWQEWDIA
PGDQILVSLAGQGIPRIDDVVWRGAERTKPTPPENRFNSLTCYFASDVCQEQFISRLVWL
GSKQVLGLDGIGEAGWRALHQTHRFEHIFSWLLLTPEQLQNTPGIAKSKSAQLWHRFNLA
RKQPFTRWVMAMGIPLTRAALNASDERSWSQLLFSTEQFWQQLPGTGSGRARQVIEWKEN
AQIKKLGSWLAAQQITGFEP