Protein Info for MCAODC_04430 in Escherichia coli ECRC101

Name: frvB
Annotation: PTS fructose-like transporter subunit IIBC

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 479 transmembrane" amino acids 132 to 160 (29 residues), see Phobius details amino acids 173 to 198 (26 residues), see Phobius details amino acids 205 to 222 (18 residues), see Phobius details amino acids 228 to 247 (20 residues), see Phobius details amino acids 259 to 281 (23 residues), see Phobius details amino acids 301 to 320 (20 residues), see Phobius details amino acids 340 to 360 (21 residues), see Phobius details amino acids 372 to 392 (21 residues), see Phobius details amino acids 399 to 419 (21 residues), see Phobius details amino acids 439 to 462 (24 residues), see Phobius details TIGR00829: PTS system, Fru family, IIB component" amino acids 8 to 94 (87 residues), 102.1 bits, see alignment E=1.7e-33 PF02302: PTS_IIB" amino acids 9 to 101 (93 residues), 73.8 bits, see alignment E=1.5e-24 TIGR01427: PTS system, Fru family, IIC component" amino acids 124 to 448 (325 residues), 185.6 bits, see alignment E=1.6e-58 PF02378: PTS_EIIC" amino acids 134 to 402 (269 residues), 115.2 bits, see alignment E=3.3e-37

Best Hits

Swiss-Prot: 99% identical to PTFLB_ECOLI: Fructose-like PTS system EIIBC component (frvB) from Escherichia coli (strain K12)

KEGG orthology group: K11202, PTS system, fructose-specific IIB-like component [EC: 2.7.1.69] K11203, PTS system, fructose-specific IIC-like component (inferred from 100% identity to ecf:ECH74115_5350)

MetaCyc: 99% identical to putative PTS enzyme IIBC component FrvB (Escherichia coli K-12 substr. MG1655)

Predicted SEED Role

"PTS system, fructose-specific IIB component (EC 2.7.1.69) / PTS system, fructose-specific IIC component (EC 2.7.1.69)" in subsystem Fructose utilization (EC 2.7.1.69)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.1.69

Use Curated BLAST to search for 2.7.1.69

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (479 amino acids)

>MCAODC_04430 PTS fructose-like transporter subunit IIBC (Escherichia coli ECRC101)
MESSLRIVAITNCPAGIAHTYMVAEALEQKARSLGHTIKVETQGSSGVENRLSSEEIAAA
DYVILATGRGLSGDDRARFAGKKVYEIAISQALKNIDQIFSELPTNSQLFAADSGVKLGK
QEVQSGSVMSHLMAGVSAALPFVIGGGILVALANMLVQFGLPYTDMSKGAPSFTWVVESI
GYLGFTFMIPIMGAYIASSIADKPAFAPAFLVCYLANDKALLGTQSGAGFLGAVVLGLAI
GYFVFWFRKVRLGKALQPLLGSMLIPFVTLLVFGVLTYYVIGPVMSDLMGGLLHFLNTIP
PSMKFAAAFLVGAMLAFDMGGPINKTAWFFCFSLLEKHIYDWYAIVGVVPVAAGLATFIA
PKLFTRQEKEAASSAIVVGATVATEPAIPYALAAPLPMITANTLAGGITGVLVIAFGIKR
LAPGLGIFDPLIGLMSPVGSFYLVLAIGLALNISFIIVLKGLWLRRKAKAAQQELVHEH