Protein Info for MCAODC_03420 in Escherichia coli ECRC101

Name: aphA
Annotation: acid phosphatase AphA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01672: HAD phosphatase, family IIIB
  • amino acids 1 to 237 (237 residues), 421.4 bits, E=5.1e-131
• PF03767: Acid_phosphat_B
  • amino acids 16 to 219 (204 residues), 102.4 bits, E=1.4e-33

Best Hits

Swiss-Prot: 99% identical to APHA_ECOLI: Class B acid phosphatase (aphA) from Escherichia coli (strain K12)

KEGG orthology group: K03788, acid phosphatase (class B) [EC: 3.1.3.2] (inferred from 99% identity to eco:b4055)

MetaCyc: 99% identical to acid phosphatase / phosphotransferase (Escherichia coli K-12 substr. MG1655)
RXN0-3741 [EC: 3.1.3.89]; 3'-nucleotidase. [EC: 3.1.3.89, 3.1.3.6]; 5'-nucleotidase. [EC: 3.1.3.89, 3.1.3.6, 3.1.3.5]; Deoxynucleotide 3'-phosphatase. [EC: 3.1.3.89, 3.1.3.6, 3.1.3.5, 3.1.3.34]; 3.1.3.34 [EC: 3.1.3.89, 3.1.3.6, 3.1.3.5, 3.1.3.34]; 3.1.3.34 [EC: 3.1.3.89, 3.1.3.6, 3.1.3.5, 3.1.3.34]; 3.1.3.34 [EC: 3.1.3.89, 3.1.3.6, 3.1.3.5, 3.1.3.34]; 3.1.3.34 [EC: 3.1.3.89, 3.1.3.6, 3.1.3.5, 3.1.3.34]; Phosphoserine phosphatase. [EC: 3.1.3.89, 3.1.3.6, 3.1.3.5, 3.1.3.34, 3.1.3.3]; Acid phosphatase. [EC: 3.1.3.89, 3.1.3.6, 3.1.3.5, 3.1.3.34, 3.1.3.3, 3.1.3.2]; 3.1.3.2 [EC: 3.1.3.89, 3.1.3.6, 3.1.3.5, 3.1.3.34, 3.1.3.3, 3.1.3.2]; 3.1.3.- [EC: 3.1.3.89, 3.1.3.6, 3.1.3.5, 3.1.3.34, 3.1.3.3, 3.1.3.2]; RXN-14534 [EC: 3.1.3.89, 3.1.3.6, 3.1.3.5, 3.1.3.34, 3.1.3.3, 3.1.3.2]

Predicted SEED Role

"NMN phosphatase (EC 3.1.3.5); Class B acid phosphatase precursor (EC 3.1.3.2)" (EC 3.1.3.2, EC 3.1.3.5)

MetaCyc Pathways

• purine nucleotides degradation II (aerobic) (11/11 steps found)
• superpathway of sulfate assimilation and cysteine biosynthesis (9/9 steps found)
• adenosine nucleotides degradation II (5/5 steps found)
• purine nucleotides degradation I (plants) (10/12 steps found)
• adenosine nucleotides degradation I (7/8 steps found)
• guanosine nucleotides degradation II (4/4 steps found)
• guanosine nucleotides degradation III (4/4 steps found)
• inosine 5'-phosphate degradation (4/4 steps found)
• superpathway of L-serine and glycine biosynthesis I (4/4 steps found)
• L-serine biosynthesis I (3/3 steps found)
• NAD salvage pathway III (to nicotinamide riboside) (3/3 steps found)
• superpathway of guanosine nucleotides degradation (plants) (5/6 steps found)
• phosphate acquisition (1/1 steps found)
• guanosine nucleotides degradation I (3/4 steps found)
• superpathway of purines degradation in plants (13/18 steps found)
• UTP and CTP dephosphorylation I (5/7 steps found)
• ureide biosynthesis (5/7 steps found)
• NAD salvage (plants) (5/11 steps found)
• tunicamycin biosynthesis (2/9 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• Glycine, serine and threonine metabolism
• Nicotinate and nicotinamide metabolism
• Purine metabolism
• Pyrimidine metabolism
• Riboflavin metabolism
• gamma-Hexachlorocyclohexane degradation

Isozymes

Compare fitness of predicted isozymes for: 3.1.3.2, 3.1.3.3, 3.1.3.5

Use Curated BLAST to search for 3.1.3.2 or 3.1.3.3 or 3.1.3.34 or 3.1.3.5 or 3.1.3.6 or 3.1.3.89

Protein Sequence (237 amino acids)

>MCAODC_03420 acid phosphatase AphA (Escherichia coli ECRC101)
MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQAPIHWVSVAQIENSLAG
RPPMAVGFDIDDTVLFSSPGFWRGKKNFSPESEDYLKNPVFWEKMNNGWDEFSIPKEVAR
QLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPATSMNPVIFAGDKPGQNTKSQW
LQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTYKPLPQAGAFGEEVIVNSEY