Protein Info for MCAODC_01720 in Escherichia coli ECRC101

Name: lplA
Annotation: YtjB family periplasmic protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 562 signal peptide" amino acids 1 to 30 (30 residues), see Phobius details transmembrane" amino acids 164 to 184 (21 residues), see Phobius details PF10144: SMP_2" amino acids 1 to 160 (160 residues), 223.8 bits, see alignment E=1.4e-70 TIGR00545: lipoyltransferase and lipoate-protein ligase" amino acids 225 to 553 (329 residues), 468.7 bits, see alignment E=4.8e-145 PF03099: BPL_LplA_LipB" amino acids 277 to 381 (105 residues), 36.2 bits, see alignment E=8.3e-13 PF10437: Lip_prot_lig_C" amino acids 474 to 557 (84 residues), 64.3 bits, see alignment E=1.3e-21

Best Hits

KEGG orthology group: K03800, lipoate-protein ligase A [EC: 2.7.7.63] K07186, membrane protein (inferred from 100% identity to etw:ECSP_5469)

Predicted SEED Role

"Lipoate-protein ligase A" in subsystem Lipoic acid metabolism

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.7.63

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (562 amino acids)

>MCAODC_01720 YtjB family periplasmic protein (Escherichia coli ECRC101)
MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQLEELARTLARQVTLNV
APLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIARSGESVEVRDRLALDGKKA
GGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDNTTNILRLMLLLSLAIGVVLT
RTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKECPITTRKEIVMSTLRLLISDSYDPWF
NLAVEECIFRQMPATQRVLFLWRNADTVVIGRAQNPWKECNTRRMEEDNVRLARRSSGGG
AVFHDLGNTCFTFMAGKPEYDKTISTSIVLNALNALGVSAEASGRNDLVVKTAEGDRKVS
GSAYRETKDRGFHHGTLLLNADLSRLANYLNPDKKKLAAKGITSVRSRVTNLTELLPGIP
HEQVCEAITEAFFAHYGERVEAEIISPDKTPDLPNFAETFARQSSWEWNFGQAPAFSHLL
DERFSWGGVELHFDVEKGHITRAQVFTDSLNPAPLEALAGRLQGCLYRADMLQQECEALL
VDFPDQEKELRELSTWIAGAVR