Protein Info for MCAODC_01400 in Escherichia coli ECRC101

Name: fixX
Annotation: ferredoxin-like protein FixX

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 428 signal peptide" amino acids 1 to 26 (26 residues), see Phobius details PF01494: FAD_binding_3" amino acids 7 to 184 (178 residues), 48 bits, see alignment E=6.8e-16 PF12831: FAD_oxidored" amino acids 7 to 157 (151 residues), 39.4 bits, see alignment E=3e-13 PF01134: GIDA" amino acids 7 to 162 (156 residues), 32.9 bits, see alignment E=2.3e-11 PF01266: DAO" amino acids 7 to 54 (48 residues), 33.7 bits, see alignment 1.9e-11 amino acids 110 to 329 (220 residues), 36.5 bits, see alignment E=2.5e-12 PF00890: FAD_binding_2" amino acids 7 to 41 (35 residues), 25.9 bits, see alignment 3.4e-09 PF03486: HI0933_like" amino acids 7 to 165 (159 residues), 33.4 bits, see alignment E=1.3e-11 PF13450: NAD_binding_8" amino acids 10 to 42 (33 residues), 31.6 bits, see alignment (E = 9.5e-11) PF21162: ETFQO_UQ-bd" amino acids 182 to 280 (99 residues), 35.7 bits, see alignment E=6.4e-12

Best Hits

Swiss-Prot: 100% identical to FIXC_ECO57: Protein FixC (fixC) from Escherichia coli O157:H7

KEGG orthology group: K00313, electron transfer flavoprotein-quinone oxidoreductase [EC: 1.5.5.-] (inferred from 100% identity to eco:b0043)

Predicted SEED Role

"Probable electron transfer flavoprotein-quinone oxidoreductase FixC (EC 1.5.5.-)" in subsystem Acetyl-CoA fermentation to Butyrate (EC 1.5.5.-)

Isozymes

Compare fitness of predicted isozymes for: 1.5.5.-

Use Curated BLAST to search for 1.5.5.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (428 amino acids)

>MCAODC_01400 ferredoxin-like protein FixX (Escherichia coli ECRC101)
MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKNVTGGRLYAHSLEHIIP
GFAESAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRSYSVLRSKFDAWLMEQAEE
AGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVILADGVNSILAEKLGMAKRVKPTN
VAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTDGLMGGGFLYTNENTLSLGLVCG
LHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEYSAHVVPEAGINMLPELVGDGVLIA
GDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSAMKSDDFSKQKLAEYRQHLESGPLRDM
RMYQKLPAFLDNPRMFSGYPELAVGVARDLFTIDGSAPELMRKKILRHGKKVGFINLIKD
GMKGVTVL