Protein Info for MCAODC_00750 in Escherichia coli ECRC101

Name: glnD
Annotation: bifunctional uridylyltransferase/uridylyl-removing protein GlnD

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 890 PF03445: DUF294" amino acids 43 to 125 (83 residues), 23.9 bits, see alignment E=8.2e-09 TIGR01693: protein-P-II uridylyltransferase" amino acids 51 to 884 (834 residues), 1004.2 bits, see alignment E=2.8e-306 PF01909: NTP_transf_2" amino acids 85 to 143 (59 residues), 29.5 bits, see alignment 2e-10 PF08335: GlnD_UR_UTase" amino acids 193 to 331 (139 residues), 156.4 bits, see alignment E=1.2e-49 PF01966: HD" amino acids 468 to 601 (134 residues), 47.9 bits, see alignment E=3.9e-16 PF01842: ACT" amino acids 709 to 762 (54 residues), 33.7 bits, see alignment 6e-12 amino acids 817 to 874 (58 residues), 36.9 bits, see alignment 6.4e-13

Best Hits

Swiss-Prot: 100% identical to GLND_ECO5E: Bifunctional uridylyltransferase/uridylyl-removing enzyme (glnD) from Escherichia coli O157:H7 (strain EC4115 / EHEC)

KEGG orthology group: K00990, [protein-PII] uridylyltransferase [EC: 2.7.7.59] (inferred from 99% identity to ecg:E2348C_0172)

Predicted SEED Role

"[Protein-PII] uridylyltransferase (EC 2.7.7.59)" in subsystem Ammonia assimilation (EC 2.7.7.59)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.7.59

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (890 amino acids)

>MCAODC_00750 bifunctional uridylyltransferase/uridylyl-removing protein GlnD (Escherichia coli ECRC101)
MNTLPEQYANTALPTLPGQPQNPCVWPRDELTVGGIKAHIDTFQRWLGDAFDNGISAEQL
IEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHPLSDIDLLILSRKKLPDDQ
AQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVATNLIESRLLIGDVALFLELQK
HIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPDIKSSPGGLRDIHTLQWVARRHF
GATSLDEMVGFGFLTSAERAELNECLHILWRIRFALHLVVSRYDNRLLFDRQLSVAQRLN
YSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLFDEAILALPADEKPRPIDDEFQLRGTL
IDLRDETLFMRQPEAILRMFYTMVRNSAITGIYSTTLRQLRHARRHLQQPLCNIPQARKL
FLSILRHPGAVRRGLLPMHRHSVLGAYMPQWSHIVGQMQFDLFHAYTVDEHTIRVMLKLE
SFASEETRQRHPLCVNVWPRLPSTELIFIAALFHDIAKGRGGDHSILGAQDVVHFAELHG
LNSRETQLVAWLVRQHLLMSVTAQRRDIQDPEVIKQFAEEVQTENRLRYLVCLTVADICA
TNETLWNSWKQSLLRELYFATEKQLRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEVL
HQIWSRCRANYFVRHSPNQLAWHARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYL
FAAVCAELDRRNLSVHDAQIFTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQ
SSWQPPQPRRQPAKLRHFTVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGI
SLHGARITTIGERVEDLFIIATADRRALNNELQQEVHQRLTEALNPNDKG