Protein Info for MCAODC_00240 in Escherichia coli ECRC101

Name: yafO
Annotation: type II toxin-antitoxin system mRNA interferase toxin YafO

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 132 PF13957: YafO_toxin" amino acids 27 to 130 (104 residues), 131.3 bits, see alignment E=6.4e-43

Best Hits

Swiss-Prot: 98% identical to YAFO_ECOLI: mRNA interferase toxin YafO (yafO) from Escherichia coli (strain K12)

KEGG orthology group: None (inferred from 98% identity to eco:b0233)

MetaCyc: 98% identical to ribosome-dependent mRNA interferase toxin YafO (Escherichia coli K-12 substr. MG1655)
3.1.26.-

Predicted SEED Role

"Toxin YafO"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (132 amino acids)

>MCAODC_00240 type II toxin-antitoxin system mRNA interferase toxin YafO (Escherichia coli ECRC101)
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAH
IHLANVNNPFPPQLRQFSRTNDESHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHK
IGKMAEAFRMRF