Protein Info for LU632_RS24145 in Erwinia tracheiphila SCR3

Name: dapF
Annotation: diaminopimelate epimerase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00652: diaminopimelate epimerase
  • amino acids 1 to 273 (273 residues), 336.5 bits, E=6e-105
• PF01678: DAP_epimerase
  • amino acids 3 to 123 (121 residues), 110.9 bits, E=2e-36
  • amino acids 152 to 267 (116 residues), 126.1 bits, E=4.2e-41

Best Hits

Swiss-Prot: 93% identical to DAPF_ERWT9: Diaminopimelate epimerase (dapF) from Erwinia tasmaniensis (strain DSM 17950 / CIP 109463 / Et1/99)

KEGG orthology group: K01778, diaminopimelate epimerase [EC: 5.1.1.7] (inferred from 93% identity to eta:ETA_02150)

MetaCyc: 87% identical to diaminopimelate epimerase (Escherichia coli K-12 substr. MG1655)
Diaminopimelate epimerase. [EC: 5.1.1.7]

Predicted SEED Role

"Diaminopimelate epimerase (EC 5.1.1.7)" in subsystem Lysine Biosynthesis DAP Pathway (EC 5.1.1.7)

MetaCyc Pathways

• superpathway of L-lysine, L-threonine and L-methionine biosynthesis I (17/18 steps found)
• aspartate superpathway (21/25 steps found)
• L-lysine biosynthesis I (9/9 steps found)
• superpathway of L-lysine, L-threonine and L-methionine biosynthesis II (13/15 steps found)
• L-lysine biosynthesis VI (6/7 steps found)
• L-lysine biosynthesis II (6/9 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
• Lysine biosynthesis

Isozymes

No predicted isozymes

Use Curated BLAST to search for 5.1.1.7

Protein Sequence (274 amino acids)

>LU632_RS24145 diaminopimelate epimerase (Erwinia tracheiphila SCR3)
MQFSKMHGLGNDFMVVDAVTQNVYFSPELIRRLADRHLGIGFDQLLIVEPPYDPDLDFHY
RIFNADGSEVAQCGNGARCFARFVRLKGLTNKSDIHVSTHTGRMVLSVTEDELVCVNMGE
PNFEPQQVPFRANKAENLYLMRAAEQTVMCGVVSMGNPHCVLQVDSVQAAQVETLGPVLE
SHERFPERVNVGFMEVVSREYIRLRVYERGAGETQACGSGACAAVAVGIQQAQLAEKVRV
DLPGGRLDIAWKGAGQPLYMTGPATHVYDGFIHL