Protein Info for LU632_RS24005 in Erwinia tracheiphila SCR3

Name: ubiE
Annotation: bifunctional demethylmenaquinone methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol methylase UbiE

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 251 PF01209: Ubie_methyltran" amino acids 19 to 250 (232 residues), 345.7 bits, see alignment E=3.9e-107 TIGR01934: ubiquinone/menaquinone biosynthesis methyltransferase" amino acids 25 to 250 (226 residues), 290.7 bits, see alignment E=3e-91 PF13489: Methyltransf_23" amino acids 57 to 234 (178 residues), 53.4 bits, see alignment E=9.3e-18 PF01135: PCMT" amino acids 61 to 146 (86 residues), 23.8 bits, see alignment E=1.2e-08 PF13847: Methyltransf_31" amino acids 63 to 225 (163 residues), 78.7 bits, see alignment E=1.4e-25 PF13649: Methyltransf_25" amino acids 67 to 164 (98 residues), 81.2 bits, see alignment E=2.6e-26 PF08242: Methyltransf_12" amino acids 68 to 166 (99 residues), 46.1 bits, see alignment E=2.4e-15 PF08241: Methyltransf_11" amino acids 68 to 167 (100 residues), 75.9 bits, see alignment E=1.2e-24

Best Hits

Swiss-Prot: 90% identical to UBIE_ERWT9: Ubiquinone/menaquinone biosynthesis C-methyltransferase UbiE (ubiE) from Erwinia tasmaniensis (strain DSM 17950 / CIP 109463 / Et1/99)

KEGG orthology group: K03183, ubiquinone/menaquinone biosynthesis methyltransferase [EC: 2.1.1.- 2.1.1.163] (inferred from 92% identity to ebi:EbC_02330)

MetaCyc: 81% identical to bifunctional 2-octaprenyl-6-methoxy-1,4-benzoquinol methylase and demethylmenaquinone methyltransferase (Escherichia coli K-12 substr. MG1655)
2-OCTAPRENYL-METHOXY-BENZOQ-METH-RXN [EC: 2.1.1.201]; ADOMET-DMK-METHYLTRANSFER-RXN [EC: 2.1.1.201, 2.1.1.163]

Predicted SEED Role

"Ubiquinone/menaquinone biosynthesis methyltransferase UbiE (EC 2.1.1.-) @ 2-heptaprenyl-1,4-naphthoquinone methyltransferase (EC 2.1.1.163)" (EC 2.1.1.-, EC 2.1.1.163)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.1.1.-

Use Curated BLAST to search for 2.1.1.- or 2.1.1.163 or 2.1.1.201

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (251 amino acids)

>LU632_RS24005 bifunctional demethylmenaquinone methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol methylase UbiE (Erwinia tracheiphila SCR3)
MADESKDTTHFGYRTVAKSDKAEMVADVFHSVAAKYDLMNDLMSFGIHRIWKRFTIDASG
VRRGQRVLDLAGGTGDLTAKFSRLVGDTGEVVLADINSSMLKVGREKLRNKGIIGNINYV
QANAEALPFPDNHFDCITIAFGLRNVTDKDKALASMFRVLKPSGRLLVLEFSKPVLAPLS
KIYDAYSFHVLPRIGELVAKDSESYRYLAESIRMHPDQDTLKQMMTDVGFENITYSNFTG
GIVALHRGFKF