Protein Info for LU632_RS21355 in Erwinia tracheiphila SCR3

Annotation: Do family serine endopeptidase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 455 signal peptide" amino acids 1 to 36 (36 residues), see Phobius details TIGR02037: peptidase Do" amino acids 40 to 453 (414 residues), 516 bits, see alignment E=4.4e-159 PF00089: Trypsin" amino acids 95 to 255 (161 residues), 72.8 bits, see alignment E=9.2e-24 PF13365: Trypsin_2" amino acids 96 to 232 (137 residues), 115.6 bits, see alignment E=8.2e-37 PF00595: PDZ" amino acids 266 to 349 (84 residues), 53.5 bits, see alignment E=6.4e-18 amino acids 389 to 443 (55 residues), 39.2 bits, see alignment 1.8e-13 PF13180: PDZ_2" amino acids 282 to 361 (80 residues), 54 bits, see alignment E=4.3e-18 amino acids 390 to 449 (60 residues), 33 bits, see alignment E=1.5e-11 PF17820: PDZ_6" amino acids 297 to 351 (55 residues), 38.4 bits, see alignment 2.2e-13 amino acids 394 to 435 (42 residues), 35.2 bits, see alignment 2.1e-12

Best Hits

Swiss-Prot: 67% identical to DEGQ_ECOLI: Periplasmic pH-dependent serine endoprotease DegQ (degQ) from Escherichia coli (strain K12)

KEGG orthology group: K04772, serine protease DegQ [EC: 3.4.21.-] (inferred from 79% identity to ebi:EbC_40550)

MetaCyc: 67% identical to periplasmic serine endoprotease (Escherichia coli K-12 substr. MG1655)
3.4.21.-

Predicted SEED Role

"Outer membrane stress sensor protease DegQ, serine protease" in subsystem Proteolysis in bacteria, ATP-dependent

Isozymes

Compare fitness of predicted isozymes for: 3.4.21.-

Use Curated BLAST to search for 3.4.21.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (455 amino acids)

>LU632_RS21355 Do family serine endopeptidase (Erwinia tracheiphila SCR3)
MKKKFLLLSALALSTGLSMAATPMAMAALPAQIQGQPMPSLAPMLEKVLPAVVSVHVEGT
QSQPQTQDIPEPLKRFFGQQDQNNEAAQPQPFEGLASGVIINADKGYILTNNHVINGADK
ISVQLSDGREFDAKLIGHDQPTDIALLQVSGAKGLAQIKIADSDQLKVGDFAVAIGNPFG
LGQTATSGIISALGRSGLNLEGLENFIQTDAAINRGNSGGALVNLNGELIGINTAILASS
GGNIGIGFAIPGNMAMNLAQQLIDFGEVRRGQLGIKGTEMTAEMAKTFKVDAQRGAFVNE
VLPGSAALKAGIKAGDIITHLDDKPVESFAALRAKIGTTAPGKEVKLELLRDGKPMTLMV
RLDISSQTISSAEEIAPAFQGSTLSNAAARGVNVDDVSKGTPAEQSGLQKGDVIIGLNRS
PVGNLTELRKLLATKPPLLALNIIRGDQTIYLLLR