Protein Info for LU632_RS19870 in Erwinia tracheiphila SCR3

Name: tilS
Annotation: tRNA lysidine(34) synthetase TilS

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 446 PF01171: ATP_bind_3" amino acids 15 to 193 (179 residues), 196.9 bits, see alignment E=4e-62 TIGR02432: tRNA(Ile)-lysidine synthetase" amino acids 16 to 197 (182 residues), 173.8 bits, see alignment E=3.7e-55 PF09179: TilS" amino acids 242 to 309 (68 residues), 67 bits, see alignment E=2.2e-22 TIGR02433: tRNA(Ile)-lysidine synthetase, C-terminal domain" amino acids 370 to 415 (46 residues), 52.9 bits, see alignment 1.9e-18 PF11734: TilS_C" amino acids 370 to 441 (72 residues), 79.7 bits, see alignment E=1.2e-26

Best Hits

Predicted SEED Role

"tRNA(Ile)-lysidine synthetase (EC 6.3.4.19)" (EC 6.3.4.19)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.4.19

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (446 amino acids)

>LU632_RS19870 tRNA lysidine(34) synthetase TilS (Erwinia tracheiphila SCR3)
MSLLNLQQQLDEHQQLLVAYSGGLDSTVLLHQLVCLRQQLPGIHLRAIHIHHGLNAHADS
WVNHCQQQCAEWQVLFKVVHVRLDTRGKGVEAAARDARYQAFLENLQPGEVLLTAQHQND
QCETLLLALKRGSGPAGLSGMSASGKLGRYLHLRPLLDFSRSQLEKWAAEHHLRWIEDDS
NQNQRYDRNFIRQAVMPLLNSRWPHFSQSVARSAALCAGQEQLLDELLSESLNALIDDRG
SLAIGPMMKMSEARRSALIRRWIASQQGLMPSRDALLRIWHEIACSREDAEPRLRLGGNE
IRRYRDRLYWLELCHSCHDVEIEWPPQQKSLALSVSGGLLVRTTYSARGTKEDKSSQSVI
VRPPLMQERVTIRFQAHGKFHIVGRAGGRSLKKILQESAIPPWQRGRIPMIFYNDCLIAA
VGVFVTREGDPGTELGWQIRWQHDLA