Protein Info for LU632_RS19735 in Erwinia tracheiphila SCR3

Name: mqo
Annotation: malate dehydrogenase (quinone)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF06039: Mqo
  • amino acids 19 to 507 (489 residues), 779.2 bits, E=1.4e-238
• TIGR01320: malate dehydrogenase (acceptor)
  • amino acids 20 to 505 (486 residues), 691.3 bits, E=3.5e-212
• PF01266: DAO
  • amino acids 21 to 293 (273 residues), 37.7 bits, E=1.8e-13

Best Hits

Swiss-Prot: 68% identical to MQO_ENT38: Probable malate:quinone oxidoreductase (mqo) from Enterobacter sp. (strain 638)

KEGG orthology group: K00116, malate dehydrogenase (quinone) [EC: 1.1.5.4] (inferred from 78% identity to ebi:EbC_01410)

MetaCyc: 67% identical to malate:quinone oxidoreductase (Escherichia coli K-12 substr. MG1655)
MALATE-DEHYDROGENASE-ACCEPTOR-RXN [EC: 1.1.5.4]

Predicted SEED Role

"Malate:quinone oxidoreductase (EC 1.1.5.4)" in subsystem TCA Cycle (EC 1.1.5.4)

MetaCyc Pathways

• superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass (23/26 steps found)
• TCA cycle I (prokaryotic) (9/10 steps found)
• partial TCA cycle (obligate autotrophs) (7/8 steps found)
• TCA cycle VI (Helicobacter) (7/9 steps found)
• superpathway of glyoxylate bypass and TCA (9/12 steps found)
• TCA cycle VII (acetate-producers) (6/9 steps found)

KEGG Metabolic Maps

• Pyruvate metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.1.5.4

Use Curated BLAST to search for 1.1.5.4

Protein Sequence (529 amino acids)

>LU632_RS19735 malate dehydrogenase (quinone) (Erwinia tracheiphila SCR3)
MRKSAESLTPTSVQDQKNDVDVLLIGGGIMSATLGAYLQELEPGWHISMVERLEDVAGES
SDGWNNAGTGHAALAEMNYTPEKSDGSVDIQKAININEAFQLSRQFWAHQVQRGMLHTPH
SFIKSVPHMSFVWGDENIRFLKKRFSALQKSTLFRGMEYSEDTEQIRKWVPLMMEGRDAD
QRVAATRISSGTDVNFGDITRQLIASLQNTSRFTLQTRHEVRRFKRNQDGSWNVILADLK
NNGNEKLLQAKYIFIGAGGAALTLLQGTGIPEAKNYAGFPVGGEFLVTDNPQVVSRHMAK
VYGKASVGAPPMSVPHLDTRMLDGKQVLLFGPFATFSTRFLKQGSLWDMFGSITPTNLMP
MMHVGLGNIDLIKYLVGQMMLTDDARLKALQEYFPEARKEDWRRVKAGQRVQIIKKDEKK
GGVLKLGTEVVTSEDGTVSALLGASPGASTAAPIMLDLMNKVFKDKFHSTEWQQKLKAII
PSYDQKLNGNIAATEHELAETSRILQLDYTPSSAANDENSDDREAVIGK