Protein Info for LU632_RS19005 in Erwinia tracheiphila SCR3

Annotation: beta-galactosidase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1024 PF02837: Glyco_hydro_2_N" amino acids 57 to 218 (162 residues), 187 bits, see alignment E=7e-59 PF00703: Glyco_hydro_2" amino acids 221 to 333 (113 residues), 48.9 bits, see alignment E=2.7e-16 PF02836: Glyco_hydro_2_C" amino acids 335 to 628 (294 residues), 377.8 bits, see alignment E=8.8e-117 PF16353: LacZ_4" amino acids 640 to 724 (85 residues), 58.2 bits, see alignment E=2e-19 PF02929: Bgal_small_N" amino acids 753 to 1020 (268 residues), 188.3 bits, see alignment E=5.2e-59

Best Hits

Swiss-Prot: 60% identical to BGAL1_KLEP7: Beta-galactosidase 1 (lacZ1) from Klebsiella pneumoniae subsp. pneumoniae (strain ATCC 700721 / MGH 78578)

KEGG orthology group: K01190, beta-galactosidase [EC: 3.2.1.23] (inferred from 62% identity to ddc:Dd586_2634)

Predicted SEED Role

"Beta-galactosidase (EC 3.2.1.23)" in subsystem Galactosylceramide and Sulfatide metabolism or Lactose and Galactose Uptake and Utilization or Lactose utilization (EC 3.2.1.23)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.2.1.23

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (1024 amino acids)

>LU632_RS19005 beta-galactosidase (Erwinia tracheiphila SCR3)
MRHSPVLSLQQQLGRRDWENPVVTHINRLPAHPPFLSYRHEENARDERPDSGNRLLLNGE
WTFSYFNRPEEVPESWLRQDLPGAQRLPVPGNWQLAGFDAPIYTNVQYPIPVNPPCVPEM
NPTGCYSLNFTHDKNQLLTYIQFDGVNSAFYLWCNGEFIGYSQDSRLPAEFDLTPALRDG
SNRLAVLVLRWSDGSYLEDQDMWRMSGIFRDVSLLQKPRLHLRDVQIETHLSAEYHTGRL
QVLAICSQPDAEACQVEVILWQQTRCIARHRQSLCSQIVDERGHYRDRTTLNLTVHQPEL
WSAETPHLYRATVTLFDATGHRMETEAFDVGFRKVEIHQGLLKLNGKPLLIRGTNRHEHH
PENGQVMDEATMIRDIMLMKQHNFNAVRCSHYPAHPLWYRLCDRFGLYVVDEANIETHGM
EPMNRLSDDPLWFNAFSERFTRMVQRDRNHPCIIIWSLGNESGHGSTHDALYAWVKSSDK
SRPVQYEGGGADTAATDIICPMYARVDQDQPFEAVPKWAIKKWIGLPEETRPLILCEYAH
AMGNSLGGFAKYWQAFRAFPRLQGGFVWDWVDQSLTRYDQNGQPWQAYGGDFGDKPNDHQ
FCMNGLVFADRTPHPSLFEAQWAQQFFQFSLESSSPLRLVIRSEYLFRATDNEQLEWRIE
HNGLVISAGSQPLTLVPEGAITVELGELPLRDGELWLCVAVTQPRATAWSDAGHRVAWNQ
WRLPALLQRPSLCHDNPKPALIVDETEITIRHQQQSWHFCRKSGELTQWLQQDKALLLSP
LRDAFVRAPVDNDIGISEVTKVDPNAWVARWQRAGFNALNSELTELYYDQLSNSVQLTTV
HAWHHDGTLCFISDKQYRFNSHGEMNIQVNVTRAAEQPAPARIGLCCQLNQHDGMASWLG
LGPHENYPDRCHAAQFSRWSLPLDDLHTPYVFPCENGLRGGTKALTFGAITVTGDFHFSL
SRYSMEQLRDASHRHLLRPEEGCWLSLDGHHMGIGGDDSWSPSVSPEFLLNNARYDYAVT
FTAT