Protein Info for LU632_RS18270 in Erwinia tracheiphila SCR3

Name: amtB
Annotation: ammonium transporter AmtB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 434 signal peptide" amino acids 1 to 28 (28 residues), see Phobius details transmembrane" amino acids 38 to 61 (24 residues), see Phobius details amino acids 72 to 93 (22 residues), see Phobius details amino acids 124 to 145 (22 residues), see Phobius details amino acids 155 to 176 (22 residues), see Phobius details amino acids 189 to 211 (23 residues), see Phobius details amino acids 224 to 243 (20 residues), see Phobius details amino acids 254 to 274 (21 residues), see Phobius details amino acids 285 to 303 (19 residues), see Phobius details amino acids 309 to 330 (22 residues), see Phobius details amino acids 342 to 361 (20 residues), see Phobius details amino acids 381 to 402 (22 residues), see Phobius details TIGR00836: ammonium transporter" amino acids 37 to 432 (396 residues), 455.1 bits, see alignment E=1.1e-140 PF00909: Ammonium_transp" amino acids 39 to 432 (394 residues), 424.2 bits, see alignment E=2.3e-131

Best Hits

Swiss-Prot: 81% identical to AMTB_ECO57: Ammonia channel (amtB) from Escherichia coli O157:H7

KEGG orthology group: K03320, ammonium transporter, Amt family (inferred from 94% identity to ebi:EbC_10230)

MetaCyc: 81% identical to ammonium transporter (Escherichia coli K-12 substr. MG1655)
RXN-9615

Predicted SEED Role

"Ammonium transporter" in subsystem Ammonia assimilation

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (434 amino acids)

>LU632_RS18270 ammonium transporter AmtB (Erwinia tracheiphila SCR3)
MGWIRMKKKLAVFGLAGLALLPSLVMAATAPVVDKADNAFMMICTALVLFMTLPGIALFY
GGLIRTKNVLSMMTQVSVTFAMVCVLWMLYGYSLAFSEGNAFFGGFSMAMLKNIQLTALV
GTFYQYIHVSFQASFACITVGLIVGSIAERVRFSAVLIFVAIWLTFSYLPIAHMVWSGGL
LAQDGALDFAGGTVVHINAAVAGLVGAYLVGKRAGFGKEAFKPHNLPMVFTGTAILYVGW
FGFNAGSASAANEIAGLAFLNTVMATAGAMLSWTFGEWALRGKPSLLGVCSGAIAGLVAV
TPACGYVGVGGALIIGLVGGLAGLWGVTTLKKWLRVDDPCDVFGVHGVCGIVGCILTGVF
ASSSLGGVGYASGVTMGHQVWIQLLSVGVTIAWTGVVAFIAFKVADMIVGLRVPEDQERE
GLDVNSHGENAYNH