Protein Info for LU632_RS18115 in Erwinia tracheiphila SCR3

Name: dnaX
Annotation: DNA polymerase III subunit gamma/tau

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 646 TIGR02397: DNA polymerase III, subunit gamma and tau" amino acids 3 to 356 (354 residues), 437.8 bits, see alignment E=1.6e-135 PF13177: DNA_pol3_delta2" amino acids 20 to 178 (159 residues), 149.4 bits, see alignment E=3.9e-47 PF13401: AAA_22" amino acids 42 to 156 (115 residues), 28.4 bits, see alignment E=7.6e-10 PF00004: AAA" amino acids 42 to 170 (129 residues), 43.6 bits, see alignment E=1.7e-14 PF07728: AAA_5" amino acids 42 to 145 (104 residues), 23.2 bits, see alignment E=2.5e-08 PF12169: DNA_pol3_gamma3" amino acids 232 to 365 (134 residues), 96.9 bits, see alignment E=3.9e-31 PF12168: DNA_pol3_tau_4" amino acids 423 to 501 (79 residues), 54.7 bits, see alignment E=4.4e-18 PF12170: DNA_pol3_tau_5" amino acids 503 to 643 (141 residues), 176.8 bits, see alignment E=1e-55

Best Hits

Swiss-Prot: 70% identical to DPO3X_ECOLI: DNA polymerase III subunit tau (dnaX) from Escherichia coli (strain K12)

KEGG orthology group: K02343, DNA polymerase III subunit gamma/tau [EC: 2.7.7.7] (inferred from 81% identity to ebi:EbC_10440)

Predicted SEED Role

"DNA polymerase III subunits gamma and tau (EC 2.7.7.7)" in subsystem DNA-replication (EC 2.7.7.7)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.7.7

Use Curated BLAST to search for 2.7.7.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (646 amino acids)

>LU632_RS18115 DNA polymerase III subunit gamma/tau (Erwinia tracheiphila SCR3)
MSYQVLARKWRPQAFTDVVGQEHVLTALANGLSLGRIHHAYLFSGTRGVGKTTIARLLAK
GLNCETGITATPCGQCDNCREIEQGRFVDLIEIDAASRTKVEDTRDLLDNVQYAPARGRF
KVYLIDEVHMLSRHSFNALLKTLEEPPDHVKFLLATTDPQKLPVTILSRCLQFHLKALDA
VQIRGQLEYVLQQENISAEVRALQLLARTADGSMRDALSLTDQAIAIGAGQVTTATVIDM
LGTLDDEQPLALIEALVRAEGEKVMSLLNQAASRGVEWEALLVEMLTLLHRVAMIQLVSS
APDDEFAAAGSRLRELARVLPPAEVQLYYQTLLVGRKELPLAPDRRMGVEMTLLRALAFH
PKQLIAEPSPSAANIVSERPIPRQPEPGTETPLAVTAVTPAAVQSEPVGSLPDTTSQLLQ
ARTQLMRQQGVTKPKKSEPAAQGSQSAGLALERLAAVGERGRKRQTTPEPAEPVKKEVYR
WKSQSAEAEPEGVIATPKLLRTALEHEKTPELINKLATESELRDAWAGEIATLTLPKLVQ
QLALNAWKETLESGIVLHLRSSQRHLNSPSAQKVLREALSQLAGHPVELTLIEDDNPAVL
TPLEWRQKIYEEKLAQARQSITTDSHIQTLRQFFDADVDEESIRPI