Protein Info for LU632_RS17975 in Erwinia tracheiphila SCR3

Name: purL
Annotation: phosphoribosylformylglycinamidine synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1100 1200 1295 TIGR01735: phosphoribosylformylglycinamidine synthase" amino acids 4 to 1293 (1290 residues), 2008.4 bits, see alignment E=0 PF18076: FGAR-AT_N" amino acids 36 to 150 (115 residues), 144.3 bits, see alignment E=3.2e-46 PF18072: FGAR-AT_linker" amino acids 171 to 220 (50 residues), 80.2 bits, see alignment (E = 2.6e-26) PF02769: AIRS_C" amino acids 431 to 587 (157 residues), 120.2 bits, see alignment E=1.9e-38 amino acids 827 to 966 (140 residues), 53.3 bits, see alignment E=7.2e-18 PF13507: GATase_5" amino acids 1039 to 1292 (254 residues), 348.8 bits, see alignment E=3.2e-108

Best Hits

Swiss-Prot: 84% identical to PUR4_ECO57: Phosphoribosylformylglycinamidine synthase (purL) from Escherichia coli O157:H7

KEGG orthology group: K01952, phosphoribosylformylglycinamidine synthase [EC: 6.3.5.3] (inferred from 71% identity to aap:NT05HA_1480)

MetaCyc: 84% identical to phosphoribosylformylglycinamide synthetase (Escherichia coli K-12 substr. MG1655)
Phosphoribosylformylglycinamidine synthase. [EC: 6.3.5.3]

Predicted SEED Role

"Phosphoribosylformylglycinamidine synthase, synthetase subunit (EC 6.3.5.3) / Phosphoribosylformylglycinamidine synthase, glutamine amidotransferase subunit (EC 6.3.5.3)" in subsystem De Novo Purine Biosynthesis (EC 6.3.5.3)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.5.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (1295 amino acids)

>LU632_RS17975 phosphoribosylformylglycinamidine synthase (Erwinia tracheiphila SCR3)
MMEILRGSPALSAFRINKLLTRFQDAHLPVSDIYAEYVHFADVSAPFTADAKARLERLLK
YGPSLAGHVPEGRLLLVTPRPGTISPWSSKATDIAHNCDLPQVLRLERGLAFYVKAPQLS
ETQWTSLAALLHDRMMEVVLNELAQAEQLFVHHQPAPLHSVDISGGGRGALEEANRRLGL
ALASDEIDYLLAAFETLGRNPNDIELYMFAQANSEHCRHKIFNADWIIDGKAQPKSLFKM
IKNTFEHTPDHVLSAYKDNAAVMEGAEVGRFYTTRSGQYDFYQEETHILMKVETHNHPTA
ISPWPGAATGSGGEIRDEGATGRGAKPKAGLVGFSVSNLRIPGFEQPWEEDFGKPTHIVT
ALDIMTEGPLGGAAFNNEFGRPVLNGYFRTYEERVNSHHGPELRGYHKPIMLAGGIGNIR
ADHVKKGEISVGAKLIVLGGPAMNIGLGGGAASSMASGQSDADLDFASVQRDNPEMERRC
QEVIDRCWQLGSDNPILFIHDVGAGGLSNAMPELVSDGERGGRFNLRDIPSDEPGMSPLE
VWCNESQERYVLAVAPEKLAEFDALCQRERAPYAVIGEATEEMHLSLSDSYFNTKLIDMP
LDVLLGKTPKMTRDVATLKAKGEPLCREAIAIADAVKRVLHLPAVAEKTFLVTIGDRTVT
GMVARDQMVGPWQIPVANCAVTTASLDSYYGEAMALGERAPVALLDFAASGRLAVAEAIT
NIAATQIGSLKRIKLSANWMAAAGHPGEDAGLYEAVKAVGEELCPALGITIPVGKDSMSM
KTRWQQGSEQREMTSPLSLVITAFARVEDVRRTVTPQLQSRQDNALLLIDLGQGENALGA
TALSQVYRQLGDKPADVRDVQRLAGFFNAIQVLVSEGKLLAYHDRSDGGLLVTLAEMAFT
GHCGLEVDISALGDDVLAALFNEELGAVIQIAAGDRAAVEQCLAAHGLATCVHYMGSAQA
GDHFVITSGDTTVYSESRSTLRTWWAETTWQMQRLRDNPDCADQEHEAKKNDDDPGLNVA
LSFGPEEDIAAPFIATGVRPKVAVLREQGVNSHVEMAAAFHRAGFDAVDVHMSDLLSGRL
GLEDFQTLVACGGFSYGDVLGAGEGWAKSILFNNRVRDEFETFFHRPQTLALGVCNGCQM
MSNLHELIPGSRAWPRFVRNQSERFEARFSLVEVAASPSLLLEGMVGTRMPIAVSHGEGF
VEVRDEAHLATLEHKGLVALRFVDNFGKVTQQYPANPNGSPNGITAVSNESGCVTIMMPH
PERVFRTVSNSWHPAEWGEDSPWMRIFRNARRQLG