Protein Info for LU632_RS17800 in Erwinia tracheiphila SCR3

Name: ispG
Annotation: flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00612: 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase
  • amino acids 9 to 351 (343 residues), 413.9 bits, E=2.9e-128
• PF04551: GcpE
  • amino acids 13 to 354 (342 residues), 473 bits, E=2.7e-146

Best Hits

Swiss-Prot: 93% identical to ISPG_ERWT9: 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (flavodoxin) (ispG) from Erwinia tasmaniensis (strain DSM 17950 / CIP 109463 / Et1/99)

KEGG orthology group: K03526, (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase [EC: 1.17.7.1] (inferred from 94% identity to ebi:EbC_33760)

MetaCyc: 92% identical to (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase (flavodoxin) (Escherichia coli K-12 substr. MG1655)
RXN-15878 [EC: 1.17.7.3]

Predicted SEED Role

"1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase (EC 1.17.7.1)" in subsystem Isoprenoid Biosynthesis (EC 1.17.7.1)

MetaCyc Pathways

• superpathway of geranylgeranyl diphosphate biosynthesis II (via MEP) (12/12 steps found)
• taxadiene biosynthesis (engineered) (12/13 steps found)
• methylerythritol phosphate pathway I (9/9 steps found)
• methylerythritol phosphate pathway II (9/9 steps found)
• isoprene biosynthesis I (9/10 steps found)
• superpathway of ergosterol biosynthesis II (11/26 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from terpenoid and polyketide
• Biosynthesis of plant hormones
• Biosynthesis of terpenoids and steroids
• Terpenoid biosynthesis

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.17.7.1 or 1.17.7.3

Protein Sequence (373 amino acids)

>LU632_RS17800 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase (Erwinia tracheiphila SCR3)
MHNEALITRRKSKRIYVGKVPVGDGAPIAVQSMTNTRTTDVDATVRQIKALERVGVDIVR
VSVPTMDAAEAFHLIKQQVDVPLVADIHFDYRIALKVAEYGVDCLRINPGNIGNEERIRQ
VVDCARYNNIPIRIGVNAGSLEKDLQEKYGEPTPQALLESAMRHVDHLDRLNFDQFKVSV
KASDVFLAVESYRLLAKQIDQPLHLGITEAGGARAGSVKSAIGLGLLLSEGIGDTLRISL
AADPVEEVKVGFDILKSLRIRSRGINFIACPTCSRQEFDVIGTVNELEQRLEDIITPMDV
SIIGCVVNGPGEALVSTLGVTGSNKKSGFYEDGVRLRERLDNHDMIDQLEARIRAKAAMM
DQSRRIDVQQVEK