Protein Info for LU632_RS17315 in Erwinia tracheiphila SCR3

Name: fruA
Annotation: PTS fructose transporter subunit IIBC

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 566 transmembrane" amino acids 238 to 264 (27 residues), see Phobius details amino acids 271 to 298 (28 residues), see Phobius details amino acids 308 to 334 (27 residues), see Phobius details amino acids 354 to 374 (21 residues), see Phobius details amino acids 386 to 407 (22 residues), see Phobius details amino acids 432 to 456 (25 residues), see Phobius details amino acids 468 to 487 (20 residues), see Phobius details amino acids 494 to 516 (23 residues), see Phobius details amino acids 524 to 556 (33 residues), see Phobius details PF02302: PTS_IIB" amino acids 111 to 195 (85 residues), 72.5 bits, see alignment E=1.9e-24 TIGR00829: PTS system, Fru family, IIB component" amino acids 111 to 194 (84 residues), 131.4 bits, see alignment E=1.2e-42 TIGR01427: PTS system, Fru family, IIC component" amino acids 218 to 557 (340 residues), 485.6 bits, see alignment E=9.8e-150

Best Hits

Swiss-Prot: 78% identical to PTFBC_ECOLI: PTS system fructose-specific EIIB'BC component (fruA) from Escherichia coli (strain K12)

KEGG orthology group: K02769, PTS system, fructose-specific IIB component [EC: 2.7.1.69] K02770, PTS system, fructose-specific IIC component (inferred from 90% identity to ebi:EbC_30000)

MetaCyc: 78% identical to fructose-specific PTS multiphosphoryl transfer protein FruA (Escherichia coli K-12 substr. MG1655)

Predicted SEED Role

"PTS system, fructose-specific IIB component (EC 2.7.1.69) / PTS system, fructose-specific IIC component (EC 2.7.1.69)" in subsystem Fructose utilization (EC 2.7.1.69)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.1.69

Use Curated BLAST to search for 2.7.1.69

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (566 amino acids)

>LU632_RS17315 PTS fructose transporter subunit IIBC (Erwinia tracheiphila SCR3)
MKTLLIIDSSLGLATGYLAKNITTTAAASKGLTLTENLAEADRIIVAGKHIPTDGTLDGK
AIYLADIEQLLQEPAAVLEQAQSGAKVWHAPATADLPARSGAAAAGPKRIVAVTACPTGV
AHTFMAAEAIDAEAKKRGWWVKVETRGSVGAGNAITPEEVAAADLVIVAADIDVDLAKFA
GKPMYRTSTSLALKKTVQELDKAVAEARPYQPKGGSKSTGDAEEKKEKAGAYRHLLTGVS
YMLPMVVAGGLSIALSFAFGITAFKEPGTLAAALMQIGGGSAFALMVPVLAGFIAFSIAD
RPGLTPGLIGGMIATSINAGFLGGIIAGFIAGYAAKLISAKVKLPQSMEALKPILIIPLA
ASLITGLLMIYVVGTPVAKIMEGLTHWLANMGTANAVLLGAILGGMMCTDMGGPVNKVAY
AFGVGLLSSQTYAPMAAIMAAGMVPPLAMGLATLVARKKFNKGQQEGGKAALVLGLCFIS
EGAIPFAARDPMRVLPCCIIGGALTGAISMTIGAKLMAPHGGLFVLLIPGAITPVLGYLM
AIIAGTLVAGLAYAILKRPEAELAKA